Protocols in In Vitro Hepatocyte Research

Isolation And Culture Of Mouse Hepatocytes: Gender-Specific Gene Expression Responses To Chemical Treatments.- Cryopreservation of Hepatocytes.- Culture Conditions Promoting Hepatocyte Proliferation And Cell Cycle Synchronization.- Immortalized Human Hepatic Cell Lines For In Vitro Testing And Research Purposes.- Culture and Functional Characterization Of Human Hepatoma Hepg2 Cells.- Establishment And Characterization Of An In Vitro Model Of Fas-Mediated Hepatocyte Cell Death.- Serum-Free Directed Differentiation Of Human Embryonic Stem Cells To Hepatocytes.- Human Skin-Derived Precursor Cells: Isolation, Expansion And Hepatic Differentiation.- Generation Of Hepatocytes From Pluripotent Stem Cells For Drug Screening and Developmental Modeling.- Differentiation-Promoting Medium Additives For Hepatocyte Cultivation and Cryopreservation.- Co-Culture and Long-Term Maintenance Of Hepatocytes.- Primary Hepatocytes In Sandwich Culture.- Establishing Liver Bioreactors For In Vitro Research.- Epigenetic Modifications As Anti-Dedifferentiation Strategy For Primary Hepatocytes In Culture.- Transfection Of Primary Hepatocytes With Liver-Enriched Transcription Factors Using Adenoviral Vectors.- Transcriptomics Of Hepatocytes Treated With Toxicants For Investigating Molecular Mechanisms Underlying Hepatotoxicity.- Global Microrna Analysis In Primary Hepatocyte Cultures.- Mass Spectrometry-Based Proteomics For Relative Protein Quantification And Biomarker Identification In Primary Human Hepatocytes.- Targeted Metabolomics For Homocysteine-Related Metabolites In Primary Hepatocytes.- Measurement Of Cytochrome P450 Enzyme Induction And Inhibition In Human Hepatoma Cells.- Analysis Of Sinusoidal Drug Uptake Transporter Activities In Primary Human Hepatocytes.- Measurement Of Albumin Secretion As Functionality Test In Primary Hepatocyte Cultures.- Measurement Of Blood Coagulation Factor Synthesis In Cultures Of Human Hepatocytes.- Functionality Testing Of Primary HepatocytesIn Culture By Measuring Urea Synthesis.- Assay Of Bile Acid Conjugation And Excretion In Human Hepatocytes.- General Cytotoxicity Assessment By Means Of The MTT Assay.- Measurement Of Apoptotic And Necrotic Cell Death In Primary Hepatocyte Cultures.- Critical Factors In The Assessment Of Cholestatic Liver Injury in vitro.- In vitro Cell Culture Models Of Hepatic Steatosis.- Assessment Of Liver Fibrotic Insults in vitro.