Steroid Biochemistry

Section I. Cytochrome P450 Enzymes 1. Steroidogenic P450s (CYP11A1, 17A1, 21A2, 11B1, and 11B5)Emily Scott2. Steroid 17alpha-hydroxylase/17,20-lyase (Cytochrome P450 17A1)F. Peter Guengerich, Yasuhiro Tateishi, Kevin D. McCarty and Lu Liu Section 2. Enzymes of Estrogen Biosynthesis 3. Aromatase and Steroid SulfataseDebashi Ghosh4. Estrogenic 17b-Hydroxysteroid Dehydrogenase Section 3. Hydroxysteroid Dehydrogenases (HSD) 5. 3a-Hydroxyssteroid Dehydrogenase(s)6. Approaches to Measuring 3b-Hydroxysteroid Dehydrogenase Type 1Nima Sharifi7. 3b-Hydroxysteroid Dehydrogenase Type 28. 11b-Hydroxysteroids Dehydrogenase Type 1Alex Odermatt9. 11b-Hydroxysteroids Dehydrogenase Type 2Alex Odermatt10. 17b-Hydroxysteroid DehydrogenasesTea Lanisnik Rizner Section 4. Steroid Reductases 11. Approaches for evolutionary, biochemical, and structural analysis of bacterial steroid 5a-reductasesRen Ruobing12. Mammalian Steroid 5a-ReductaseRichard J. Auchus13. Human Steroid 5b-Reductase (AKR1D1)Trevor Martin Penning14. Human Aldo-Keto Reductases (AKR1C1-AKR1C4)-Cloning & Expression of Recombinant EnzymesTrevor Martin Penning and Andrea Detlefsen Section 5. Steroid Conjugating Enzymes 15. Steroid sulfotransferases-X SULT2A1, 2B1b and 1E1Charles Napoleon Falany16. Steroid UGT-Glucuronsyl Transfereases Section 6. Steroid Analytical Chemistry 17. Mass spectrometry of Glucocorticiids18. Mass spectrometry of Progestins19. LC-MS Quantitation of Hydroxy- and Keto-AndrogensClementina Mesaros, Andrea Detlefsen and Ryan Paulukinas20. Quantification of 11-oxygenated androgens by RP-UV-HPLC post 2,4-dinitrophenylhydrazine derivatizationRyan Paulukinas21. 11-Oxygenated AndrogensAmanda Swart22. LC-MS Quantitation of EstrogensClementina Mesaros