Opioid Receptors: Methods and Protocols
serves as a comprehensive guide to both key new techniques and established methods for the investigation of genetics, structural biology, transcription, and post-transcriptional events of opioid receptors. Other methods cover the cellular detection and trafficking of opioid receptors
in vitro
and
in vivo
. Procedures aimed to investigate signaling pathways modulated by opioid receptors and model systems to study opioid receptor-mediated functions are also included. Finally, methods to assay behavioral effects mediated by opioid receptors are described. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and authoritative,
Opioid Receptors: Methods and Protocols
will help both experienced and new entrants in this field to carry out their experiments successfully and with new inspiration.
Rezensionen / Stimmen
"Book edited by Santi M. Spampinato offers a review of state-of-the-art techniques used to analyze opioid receptors functionality on molecular, cellular, structural, and organism level. . The book is aimed for practicing researchers actively engaged in the field of opioid receptors investigation. Everyone from graduate students to principal investigator may find it useful." (Lubica Lacinova, General Physiology and Biophysics, Vol. 34 (3), 2015)
Reihe
Auflage
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
30
26 farbige Abbildungen, 30 s/w Abbildungen
XV, 326 p. 56 illus., 26 illus. in color.
ISBN-13
978-1-4939-1708-2 (9781493917082)
DOI
10.1007/978-1-4939-1708-2
Schweitzer Klassifikation
Overview of Genetic Analysis of Human Opioid Receptors.- Computational Structural Biology of Opioid Receptors.- Analysis of Epigenetic Mechanisms Regulating Opioid Receptor Gene Transcription.- Renilla Luciferase Reporter Assay to Study 3'UTR-Driven Post-Transcriptional Regulations of OPRM1.- High-Throughput Gene Expression Profiling of Opioid-Induced Alterations in Discrete Brain Areas.- Real-Time Imaging of Mu Opioid Receptors by Total Internal Reflection Fluorescence Microscopy.- In Vivo Techniques to Investigate the Internalization Profile of Opioid Receptors.- Monitoring Opioid Receptor Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET).- Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Non-Visual Arrestins.- Identification and Verification of Proteins Interacting with the Kappa Opioid Receptor (KOPR).- Detection of Mu Opioid Receptor (MOPR) and Its Glycosylation in Rat and Mouse Brains by Western Blot with Anti-µC, an Affinity-Purified Polyclonal Anti-MOPR Antibody.- Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues.- [35S]GTPgS-Autoradiography for Studies of Opioid Receptor Functionality.- Fluorescence-Based, High-Throughput Assays for µ-Opioid Receptor Activation Using Membrane Potential-Sensitive Dye.- Analysis of Potassium and Calcium Imaging to Assay the Function of Opioid Receptors.- Electrophysiological Patch Clamp Assay to Monitor the Action of Opioid Receptors.- Skin-Nerve Preparation to Assay the Function of Opioid Receptors in Peripheral Endings of Sensory Neurons.- Mechanical Nociception Measurement in Mice and Rats with Automated Von Frey Equipment.- Detecting Zinc Release Induced by Mu-Opioid Receptor Agonists in Brain Slices.- Opioid Receptors: Methods for Detection and Their Modes of Actions in the Eye.- Evaluation of Murine Macrophage Cytokine Production After In Vivo Morphine Treatment.- Measurement of Macrophage Toll Like Receptor 4 Expression After Morphine Treatment.- The Role of Opioid Receptors in Migration and Wound Recovery In Vitro in Cultured Human Keratinocytes and Fibroblasts.- Role of Opioid Receptors in the Reinstatement of Opioid-Seeking Behavior: An Overview.- Analysis of Opioid-Seeking Reinstatement in the Rat.- Induction of a High Alcohol Consumption in Rats and Mice: Role of Opioid Receptors.- Evaluation of Social and Non-Social Behaviors Mediated by Opioids in Mouse Pups.
