Protein Termini Part A

1. In vitro acetyltransferase assays for NAT enzymesRonen Marmorstein2. A fluorescent CPM-based in vitro acetylation assay: a tool for assessing N-Terminal acetyltransferase activity and profiling compound activityTanja Bange3. Methods for Purification and Activity Analysis of N-Terminal AcetyltransferasesRong Huang4. N-terminal acetylation of plastid precursor proteinsSacha Baginsky5. Utilizing N-terminal acetylation specific antibodiesHenriette Aksnes6. Functional assessment of N-terminal acetyltransferase variantsNina McTiernan7. Affinity purification-mass spectrometry to identify nuclear protein interactions of N-terminal acetyltransferase NAA40Antonis Kirmizis8. A pan-N-formylmethionine-specific antibody as a tool to for analyzing Nä-terminal formylationCheol-Sang Hwang9. Chemical proteomic approaches to investigate N-myristoylationEdward W. Tate10. Analysis of the oxidation state of N-terminal methioninesSina Ghaemmaghami11. A biophysical approach to studying N-terminal cysteine oxidase substrate preferencesMark White12. Recombinant expression, purification, and characterization of human ATE1 arginyltransferaseYi Zhang13. In-bacteria arginylation assayYi Zhang14. Identification of arginylated N-termini with specific antibodiesJasper Eising15. Characterization of the autophagic N-degron pathway and monitoring its chemical modulation for therapeutic developmentYong Tae Kwon16. Developing a flow cytometric method to evaluate the stability of protein N-terminiAditya M. Kunjapur17. Degronopedia: A practical guide to identifying and targeting protein degronsWojciech Pokrzywa18. Generation and usage of ubiquitin-reference technique (URT) plasmids to validate N-degronsChang Hoon Ji19. Quantitative Insights into Protein Turnover and Ubiquitination with HiBiT and NanoBRETWojciech Pokrzywa20. Characterization of the autophagy E3 ligase/N-recognin KCMF1Chang Hoon Ji21. Identification of Ac/N-degron-recognition domain within the MARCHF6 E3 ubiquitin ligaseCheol-Sang Hwang22. TurboID technique for proximity labelling of interacting proteinsGreta Jarck23. Proteome analysis of nascent polypeptide chains using puromycinKoshi Imami24. HUNTER-DIA: An updated protocol for enrichment and mass spectrometry-based identification of protein N-terminiPitter F. Huesgen25. TERMINER - Bioinformatic detection and annotation of proteolytic protein termini in shotgun proteomics dataOliver Schilling26. Chemical proteomic approaches to investigate S-prenylationEdward W. Tate27. Quantitative analysis of C-terminal prenylated protein levels using tandem mass taggingMark Distefano28. Optimizing purification and FP-based binding assays for the E3 ligase FEM1CRong Huang