Methods in Cardiovascular and Lymphatic Biology

Isolation and purification of murine lung endothelial cells.- Two-step Positive Selection Method for the Magnetic Isolation of Lymphatic Endothelial Cells.- Isolation and Enumeration of Circulating Endothelial Cells with Antibody Labeled Staggered Herringbone Microfluidic System.- A Guided Approach to Establish a Functional Humanized Brain-on-Chip Microfluidic Model of the Neurovascular System.- Human iPSC-derived astrocyte-endothelial cell and neuron-endothelial cell co-culture models of the blood-brain barrier to study the impact of ischemic stroke in vitro.- Isolation of rodent aortic smooth muscle cells by enzymatic digestions.- Studying the vascular tone of mouse aortic rings in a standard wire myograph.- Unveiling Central Nervous System Pericytes: Immunofluorescence Protocol and Confocal Microscopy.- Serum-free Mouse Aortic Ring Assay of Angiogenesis.- A New Method to Visualize Retinal Deep Vascular Layers Using Wholemount Tissue Preparations.- Live calcium imaging on mouse primary brain endothelial cells.- Studying nitric oxide in endothelial cells.- Identifying protein-protein interaction or protein phosphorylation by Proximity Ligation Assay (PLA) in endothelial cells.- Label-free imaging with automated tracking and cell-by-cell analysis of migration, morphology, and differentiation of endothelial cells.- Assessment of angiogenic properties of malignant pleural effusion (MPE) fluid.- Ultrasound Contrast Agents in Mice: Bridging the Gap from Preclinical Research to Clinical Translation.