Epigenetics Methods, Second Edition offers an extensive overview of the tools that enhance translational epigenetics studies. This edition includes updated and revised chapters, along with newly added topics such as MALDI-TOF-MS for analyzing DNA methylation and the RIP assay for lncRNA binding. Specialists provide step-by-step guidance on methods used to study various epigenetic mechanisms. The book covers both fundamental and advanced techniques, making it an essential resource for researchers in this dynamic field. In addition to DNA methylation and histone modification techniques, the book also explores chromatin evaluation, enzyme analyses, and non-coding RNAs as epigenetic modulators.New chapters discuss recent advancements like single-cell epigenomics, epigenetic editing, and computational epigenetics. The volume addresses reproducibility issues and offers consensus-driven methods to unify approaches across experiments and labs. This edition is a vital reference for understanding and employing cutting-edge epigenetics methods.
- Provides revised and updated chapters, as well as new chapters covering recent developments
- Includes contributions by leading international investigators involved in epigenetic research and clinical and therapeutic application
- Integrates technology and translation with fundamental chapters on epigenetics methods, as well as chapters on more novel and advanced epigenetics methods
- Written at verbal and technical levels that can be understood by scientists and students alike
- Includes chapters on state-of-the-art techniques such as single-cell epigenomics, use of CRISPR/Cas9 for epigenetic editing, and epigenetics methods applied to forensics
Auflage
Sprache
Verlagsgruppe
Elsevier Science & Techn.
Dateigröße
ISBN-13
978-0-443-26760-4 (9780443267604)
Schweitzer Klassifikation
Section 1: Overview1. An Overview of Epigenetics MethodsSection 2: DNA Methylation Methods: Gene- or Locus-specific Methylation Analyses 2. Principles of Bisulfite Conversion of DNA and Methylation-specific PCR (MSP) in Biological Research3. Multiplex Methylation Specific Droplet Digital PCR (MS-ddPCR) for Detection and of Rare Methylated AllelesSection 3: DNA Methylation Methods: Global DNA Methylation and Methylomic Analyses 4. Methods for Detection of DNA Methylation in Transposable Elements5. The Illumina Infinium Methylation Assay for Genome-wide Methylation Analyses6. Methylated DNA Immunoprecipitation Sequencing: Methodological Principles and Applications7. Multiplex Analyses Using Methylation Sensitive Restriction Enzyme coupled qPCR and NGS8. Advances in Whole Genome Methylomic SequencingSection 4: DNA Methylation Methods: Additional Technologies9. Analyzing DNA Methyltransferase Enzymatic Activity: Basic Methods and Applications10. Technologies and Applications for the Assessment of 5-HydroxymethylcytosineSection 5: Histone Modification Methods11. The Chromatin Immunoprecipitation (ChIP) Assay and ChIP-qPCR12. CUT&RUN and CUT&Tag for histone modification and transcription factor binding analyses13. Deep Sequencing for Histone Modifications Employing ChIP-seq Analyses14. ChIP in Series: Sequential ChIP15. The ChIP-exo Method to Study DNA-protein interactions and Chromatin Organization at Near-Nucleotide Resolution16. ATAC-seq Method Applied to Embryonic Germ Cells and Neural Stem Cells from Mouse: Practical 17. Combinatorial ChIP and Bisulfite Sequencing (BisChIP-seq)Section 6: Epigenetic Methods for Evaluating Chromatin Higher Order 18. 3D DNA FISH for Analyses of Nuclear Chromatin ArchitectureSection 7: Enzyme Analyses of Histone Modifications19. Assessment of Histone-Modifying Enzymes Involved in Epigenetic Gene RegulationSection 8: Assessment of Non-Coding RNA as an Epigenetic Modulator20. Detection and Quantification of MicroRNAs (miRNAs) and High Throughput miRNAs Profiling21. RIP-seq in Epigenetic Analyses22. Chromatin Isolation by RNA Purification (ChIRP) and Its ApplicationsSection 9: Additional Epigenetics Methods23. Single-Cell Epigenomics: Methods and Translation24. CRISPR/Cas9 Technologies in Epigenetics Research25. Epigenome-Wide Association Study (EWAS) Methods and Applications26. Systems Biology Methods to Study the Epigenome and Metabolic- epigenetic Interactions27. Forensic Epigenetics Methods and ApplicationsSection 10: Future Directions in Epigenetics Methodology 28. Advances in Epigenetic Techniques to Study Development and Diseases
