Laccase and Polyphenol Oxidase: Biochemistry and Biotechnological Applications, a new volume in the Foundations and Frontiers in Enzymology series, provides a thorough discussion of the laccase and polyphenol oxidase enzyme families, which play a key role in the oxidation of phenolic substrates and an important biological function in many organisms. In recent years, significant advances have been made in understanding these enzymes, but few practical resources exist on their fundamental biology, research methods, and applications. In this book, leading world experts discuss the biochemistry of laccase and polyphenol oxidases, research methods, and uses across industries, offering a practical overview of these important enzyme classes, and empowering researchers to develop their own studies and hone applications. The book deftly connects topics across these intricate enzyme systems, with early chapters on physicochemical properties of PPO and laccase, followed by separate sections on these related enzyme classes' function in biological and industrial systems, production, purification, active assays, and application areas from food and wine to healthcare, bioremediation, and green and computational chemistry. Step-by-step instructions and explanation offer clear guidance in replicating key research and application methodologies discussed.
- Examines the fundamental biology of laccase and polyphenol oxidases, research methods, and their uses across industries
- Features chapter contributions from leading international experts in the field
- Offers step-by-step instructions in key research and application methodologies
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ISBN-13
978-0-443-13300-8 (9780443133008)
Schweitzer Klassifikation
ContributorsAbout the editorPrefaceCHAPTER 1 Functional roles of polyphenol oxidases in plants and fungiMehdi Kamali Dashtarzhaneh, Valentina Valencia Bernal and Fatemeh KhodadadiIntroductionPolyphenol oxidase structure and mechanismEnzymatic activity and reaction mechanisms of polyphenol oxidasesLatency and activation of polyphenol oxidasesPolyphenol oxidase and browningPolyphenol oxidase functions against biotic stress in plantsPolyphenol oxidase function against plant pathogensPolyphenol oxidase function against insect pestsFunctions of polyphenol oxidases in plants against abiotic stressBiological functions of polyphenol oxidases in plant metabolism synthesisBetalain biosynthesisAurone biosynthesisTyrosine and esculetin metabolismLignan biosynthesisFunctions of polyphenol oxidases in fungiPigmentation and browningLignin degradationMorphogenesisFungal defense and interactionPolyphenol oxidase genes and characterizationConclusionsReferencesCHAPTER 2 Plant PPO: Production, purification, and activity assaysZhao Li, Kritika Shrestha and Yu LiIntroductionOverall PPO structureStructural information on the substrate specificityPrevious investigations on plant PPO purification and characterizationCrude extraction and purification of PPOsCharacterization of plant PPOsRecombinant plant PPOs: Production, purification and characterizationPrior investigations into recombinant PPOsCase study: Recombinant production, purification and characterization of grape PPOConclusionsReferencesCHAPTER 3 Laccase and polyphenol oxidase: Biochemistry and biotechnological applicationsJ. Ashwini John, Sana Riaz, S.K. Taihid Ali and Ethiraj SelvarajanIntroductionStructure and catalytic site Mechanism of enzyme action Biochemical applications of polyphenol oxidase and laccase Biosensing/biochemical sensingRemoval of pollutants Other biotechnological applicationsFood industryTextile industryPharmaceutical industryBioremediationConclusions and future prospectivesReferencesCHAPTER 4 Recent advances in the design of PPO inhibitorsChen Wai Wong, Zhi Zhou Siew, Win Yee Lim and Eric Wei Chiang ChanIntroductionPolyphenol oxidase inhibitors in food packaging systemsThe role of food packaging on enzymatic browningActive packaging as an innovative approach to inhibit enzymatic browningChoice between plant-based and synthetic PPO inhibitors in active packagingCompatibility of plant-based inhibitors with active packagingPolyphenol oxidase inhibitors in cosmeceuticalsRole of tyrosinase in melanogenesisMelanogenesis-related dermatological issuesPlant-based cosmeceutical PPO inhibitorsDelivery systems for plant-based PPO inhibitorsEthical screening methods for melanogenesis inhibitionConclusionReferencesFurther readingCHAPTER 5 Reaction mechanism and redox potential of laccaseNabangshu Dev SharmaIntroduction - laccase as versatile biocatalystsBasic structural features of laccasesMultidomain structure of laccaseActive site characteristicsConserved sequential regionsReaction mechanism of laccases and their unique substrate binding cavitySubstrate oxidationDistinction between laccases and other MCOsOxygen reduction mechanismRedox potential of laccasesRole of the axial ligandOther impacting factorsRelation between redox potential and substrate specificityLaccase engineering and the role of redox mediatorsFactors influencing laccase activity other than redox potentialConclusionReferencesCHAPTER 6 Laccases: Biological functions and potential applicationsLan Huong Le Viet, Tomoko Matsuda and Ivanhoe K.H.
