Liposomes in Gene Delivery

Introduction Gene Therapy Strategies in Gene Delivery Disease Applications of Gene Therapy Genetic Diseases Cancer Infectious Diseases Neurological Diseases Cardiovascular Diseases Other Diseases Nucleic Acids DNA Structure and Conformation DNA Stability and Interactions Theoretical Description of DNA DNA Condensation Recombinant DNA Technology Gene Expression Promoters and Enhancers Introns and PolyA Tails Complementary DNA Cytoplasmic Gene Expression Reporter Genes Vectors Gene Delivery Naked DNA Viral Systems Nonviral Delivery Systems Liposomes Lipids and Liposome Composition Cationic Lipids Electrostatic Properties of Charged Lipids and Liposomes Cationic Liposomes Liposome Preparation Methods Mechanism of Vesicle Formation Liposome Stability Characterization and Quality Control Interactions of Liposomes with Cells and In Vivo Toxicity or Safety of Cationic Liposomes Liposomes and Cell Membranes Liposome Applications Genosomes (DNA-Lipid Complexes) Reactivity and Stability Structure of Cationic Lipid-DNA Complexes Characterization Pharmacokinetics and Biodistribution Toxicity Genosomes for Gene Delivery and Transfection Expression In Vitro Gene Transfection and Expression In Vivo Parenteral Applications of Genosomes Pulmonary Delivery Other Delivery Routes Genosome Administration into Humans Structure-Activity Relationships Structure-Activity Relationships of Cationic Lipids on the Molecular Level Structure-Activity Relationships on the Colloidal Level Mechanism of Transfection Transfection In Vitro Transfection In Vivo Factors and Steps That Determine Transfection Other Carrier Systems and Artificial Virus Polyelectrolytes Combination Complexes Liposomes in the Delivery of Antisense Oligonucleotides Introduction Antisense Oligonucleotides: Structure and Mechanism of Action Liposomes for the Delivery of Antisense Oligonucleotides An Overview of Some of the Results Ribozymes Delivery of Proteins Perspectives and Conclusion Cationic Liposomes Anionic Liposomes Sterically Stabilized Liposomes Conclusion and Afterword References Appendix A: Some Fundamental Concepts Appendix B: Periodic System of Elements Appendix C: Short Tutorial on Different Units Appendix D: Geometric Parameters of Liposomes and DNA Appendix E: Abbreviations Appendix F: Glossary Index