Immuno-oncology and immunotherapy Part A

1. Assessment of translation rate in leukemic cells and immune cells of the microenvironment by OPP protein synthesis assay Vanessa Klapp, Ozgu Gumustekin, Jérôme Paggetti, Etienne Moussay and Anne Largeot 2. Generation of transmitochondrial cybrids in cancer cells Ruth Soler-Agesta, Cristina Ripollés-Yuba, Joaquín Marco-Brualla, Raquel Moreno-Loshuertos, Ai Sato, Manuel Beltrán-Visiedo, Lorenzo Galluzzi and Alberto Anel 3. Enzyme-linked Immunospot (Elispot) assay to quantify peptide-specific IFN-¿ production by splenocytes in a mouse tumor model after radiation therapy Benoît Lecoester, Yaoyao Xie, Amélie Marguier, Laura Boulerot, Marine Malfroy, Olivier Adotévi and Jihane Boustani 4. Tumor slice culture system for ex vivo immunotherapy studies Leire Arrizabalaga, Joan Salvador Russo-Cabrera, Virginia Belsúe, Pedro Berraondo, Ignacio Melero and Fernando Aranda 5. Identification and analysis of alloreactive T lymphocytes from peripheral blood mononuclear cells Alberto Susana, Giovanni Galletti, Gabriele De Simone, Chiara Camisaschi and Enrico Lugli 6. Assessment of pDCs functional capacity upon exposure to tumor-derived soluble factors Vladimír Koucký, Linn A. Syding, Klára Placková, Lucie Pavelková and Anna Fialová 7. Degranulation assay to evaluate NK cell natural and antibody-dependent cell-mediated cytotoxicity against A549 tumor spheroids Ainara Lopez-Pardo, Ainhoa Amarilla-Irusta, Víctor Sandá, Mario Stan, Francisco Borrego and Laura Amo 8. Characterization of Natural Killer cells infiltrating irradiated murine tumors through flow cytometry Jérémy Baude, Riad Ladjohounlou, Emeric Limagne, Lisa Froidurot, Véronique Morgand and Céline Mirjolet 9. Flow cytometry detection and quantification of circulating leukocyte subpopulations in mice after brain irradiation Julie Coupey, Marine M. Leblond, Erika S. Hue and Samuel Valable 10. Flow cytometry-assisted analysis of phenotypic maturation markers on an immortalized dendritic cell line Ginevra Campia, Manuel Beltrán-Visiedo, Ruth Soler-Agesta, Ai Sato, Norma Bloy, Liwei Zhao, Peng Liu, Oliver Kepp, Guido Kroemer, Lorenzo Galluzzi and Claudia Galassi 11. Optimization and intra-assay validation of a multiparametric flow cytometric test for monitoring circulating TREGs Iole Macchia, Floriana Iacobone and Francesca Urbani 12. Cytofluorometric assessment of calreticulin exposure on CD38+ plasma cells from the human bone marrow Manuel Beltrán-Visiedo, Alfonso Serrano-Del Valle, Nelia Jiménez-Aldúan, Ruth Soler-Agesta, Javier Naval, Lorenzo Galluzzi and Isabel Marzo