Immuno-oncology and immunotherapy Part C

1. Simplified acid extraction and quantification of histones in human tumor cells Lourdes Hontecillas-Prieto, Daniel J. García-Domínguez, Rocío Flores-Campos; Juan Antonio Flores, Antonio Pérez-Pérez, Luis de la Cruz-Merino, Víctor Sánchez-Margalet and Nabil Hajji 2. Expression, purification and characterization of phosphatidylserine-targeting antibodies for biochemical and therapeutic applications Varsha Gadiyar, David Calianese, Rachael Pulica, Christopher Varsanyi, Ziren Wang, Ahmed Aquib, Alok Choudhary and Raymond B. Birge 3. In vitro ILC differentiation from human HSCs Silvia Santopolo, Cecilia Ciancaglini, Francesca Romana Mariotti, Lorenzo Moretta and Linda Quatrini 4. Optimizing protocols for human regulatory T cell isolation, expansion and functional characterization Sánchez-Moreno Inés, Martín-Otal Celia, Lasarte Juan José and Lozano Teresa 5. (1)Specific selection of stimulation-responsive ¿¿ T cells utilizing a short-term activation assay Christian Peters, Jara Simeonov, Daniel Gombert and Dieter Kabelitz 6. Generation of human and murine exhausted CD8+ T cells in vitro Rosa Ana Lacalle, Raquel Blanco, Rebeca García-Lucena and Santos Mañes 7. Ex vivo assessment of human neutrophil motility and migration Noor A.M. Bakker, Claudia Burrello and Karin E. de Visser 8. Flow cytometry-based monitoring of myeloid-derived suppressor cells in the peripheral blood of patients with solid tumors Anna-Jasmina Donaubauer, Ilka Scheer, Rainer Fietkau, Udo S. Gaipl, Benjamin Frey 9. Deciphering neutrophil heterogeneity in human blood and tumors: Methods for isolating neutrophils and assessing their effect on T-cell proliferation Nicolas Delhez, Frank Aboubakar Nana, Camille Houbion, Alexandre Bayard, Annika Bruger, Christophe Vanhaver, Sven Brandau, Pierre van der Bruggen and Thibault Hirsch 10. Splenocytes antitumor cytotoxicity assessment after prophylactic vaccination or drug treatment of tumor-bearing mice Kenny Misael Calvillo-Rodriguez, Ana Luisa Rivera-Lazarin, Reyes Tamez-Guerra, Ana Carolina Martinez-Torres and Cristina Rodriguez-Padilla 11. Therapeutic treatment of tumor-bearing mice with drug-killed cancer cells: A method to confirm immunogenic cell death and assess its therapeutic effectiveness Kenny Misael Calvillo-Rodriguez, Maria Norma Gonzalez-Flores, Reyes Tamez-Guerra,Cristina Rodriguez-Padilla, Marilena Antunes-Ricardo and Ana Carolina Martinez-Torres 12. Single-cell RNA flow cytometry to assess intratumoral production of cytokines/chemokines Khiem C. Lam and Romina S. Goldszmid 13. Multidimensional profiling of cancer microenvironments in FFPE tissues by imaging mass cytometry Marieke E. Ijsselsteijn and Noel FCC de Miranda 14. Evaluation of lymphocyte infiltration into cancer spheroids by immunofluorescent staining and 3D imaging Mireia Cruz De los Santos and Andreas Lundqvist 15. Quantitative pre-clinical imaging of hypoxia and vascularity using MRI and PET Georgia Kanli, Selma Boudissa, Radovan Jirik, Tom Adamsen, Heidi Espedal, Hans-Olav Rolfsnes, Frits Alan Thorsen, Jesus Pacheco Torres, Bassam Janji and Olivier Keunen 16. Retrovirus-based manufacturing of chimeric antigen receptor-modified T cells for cancer therapy research Sophia Stock, Luisa Fertig, Vivien D. Menkhoff, Thaddäus Strzalkowski, Manuel Caruso and Sebastian Kobold