Lysosomes

Determining lysosomal enzyme activity using flurogenic probes.- Lysosomal pH measurements via dual-imaging microscopy.- Assessing perturbations in lysosome health by flow cytometry in iPSC-derived human neuron cultures.- Label-Free Imaging of Lysosomes in Living Cells by Photothermal Microscopy.- Monitoring autophagosome biogenesis and degradation in mammalian cells using LC3B blotting.- Measurement of physiological autophagic flux in the human peripheral blood mononuclear cell pool.- Lysosome enrichment using superparamagnetic iron oxide nanoparticles (SPIONs).- Lysosomal immunoprecipitation (LysoIP) for metabolomic and proteomic analyses.- Mass spectrometry-based proteomic analysis of lysosome-enriched fractions.- Analyzing Endolysosome-Mitochondria Membrane Contact Sites by Thin Section TEM of Human Fibroblasts.- Assessment of lysosome trafficking in iPSC-derived neurons utilizing time-lapse videos and TrackMate ImageJ plugin software.- Generation of donor-specific iPSC for modelling lysosomal storage disorders.- Using CMAC Staining for Vacuole Characterization in Yeast.- Modelling lysosomal disease in Dictyostelium discoideum: Examining the trafficking and secretion of lysosomal enzymes.- Bioenergetic profiling applied to a zebrafish model of Neuronal Ceroid Lipofuscinosis, a lysosomal storage disorder.- Immunostaining methods for lysosomal storage disorder research.