Whole Cell Biocatalysis

ContributorsAbout the editorsPrefaceCHAPTER 1 Advantages and new potential applications of whole-cell biocatalysisSergio Huerta-Ochoa1 Introduction1.1 History of whole-cell biocatalysis development1.2 Technical advances and economic advantages of whole-cell biocatalysis1.3 Reaction media in whole-cell biocatalysis1.4 Main microorganisms used as whole-cell factories2 Key advances and potential applications2.1 Cell permeabilization2.2 Cell immobilization2.3 Metabolic engineering2.4 Cascade reactions2.5 Chemoenzymatic synthesis2.6 Sustainable manufacturing2.7 Pharmaceutical production2.8 Biodegradation and bioremediation2.9 Renewable energy production3 Trends and perspectivesReferencesCHAPTER 2 Reprogramming microbial cells to improve the production of biopharmaceuticals and fine chemicalsAlvaro R. Lara, Marcos López-Perez, and Francisco J. Fernández1 Introduction to molecular genetics in the production of chemical and pharmaceutical substances1.1 Significance of chemical and pharmaceutical substance production in the industry and their impact on the global economy1.2 Use of microorganisms in the production of chemical and pharmaceutical substances, with emphasis on fungi1.3 Improving fungal strains through classical genetic techniques with emphasis on antibiotics1.4 Reasons for the use of molecular genetic techniques2 Classic molecular cloning techniques2.1 Molecular cloning: A clear definition2.2 Cloning of genes and DNA fragments2.3 DNA and complementary DNA (cDNA) libraries2.4 Featured examples of molecular cloning in antibiotic production3 Gene dosage optimization3.1 Gene dosage and modulation of gene dosage3.2 Gene dosage optimization in industrial production: Importance and examples3.3 Other alternatives: E.g., increasing precursor availability and/or improving precursor and penicillin transport4 Advanced genetic engineering tools4.1 Advances in genetic engineering4.2 High-throughput sequencing (NGS) techniques4.3 Promoters and RBS (bio-bricks) libraries4.4 Synthetic biology4.5 CRISPR-Cas9 technology5 Cell factories for whole-cell biocatalysis5.1 Minimal cell factories5.2 Robust cell factories5.3 Schemes for autonomous control of the metabolic fluxes and induction of product synthesis6 The future of molecular genetics in the production of chemical and pharmaceutical substancesReferencesCHAPTER 3 Mitigation of greenhouse gas emissions from biogas-producing facilities: A novel whole-cell technology platform based on anaerobic oxidation of methaneGuillermo Quijano and Ivonne Figueroa-González1 Introduction2 GHG emissions from biogas-producing facilities3 Conventional aerobic biotechnologies for treating residual dissolved methane3.1 Aerobic methanotrophic metabolism3.2 Packed bed reactors and two-phase partitioning systems3.3 Aerobic membrane bioreactors4 Whole-cell technology platform for anaerobic methane oxidation4.1 Fundamentals and process microbiology of the N-AOM process4.2 Bioreactors and operating conditions reported for N-AOM implementation5 PerspectivesReferencesCHAPTER 4 Computational metabolic engineering using genome-scale metabolic models and constraint-based methodsCarlos Coello-Castillo, Freddy Castillo-Alfonso, and Roberto Olivares-Hernández1 Defining metabolic engineering2 Microbial cell factory3 Strategies for designing microbial cell factories4 The engineering cycle5 The principles for the calculation of metabolic fluxes6 Linear programming for metabolic network modeling7 Genome-scale mathematical modeling8 Reconstruction of the metabolic model9 Metabolic engineering and systems biology10 Data integration11 Metabolic engineering and systems biology strategiesReferencesCHAPTER 5 Whole-cell biocatalysis in nonconventional mediaDulce María Palmerín-Carreno1 Introduction2 Nonconventional media used for biocatalysis2.1 Whole-cell function in nonconventional media3 Reaction and transport mechanisms in nonconventional media3.1 Partitioning bioreactors3.