Basic Virology

Preface xxi

Acknowledgements xxvii

Part I Virology and Viral Disease 1

Chapter 1 Introduction - The Impact of Viruses on Our View of Life 3

Chapter 2 An Outline of Virus Replication and Viral Pathogenesis 15

Chapter 3 Virus Disease in Populations and Individual Animals 27

Chapter 4 Patterns of Some Viral Diseases of Humans 43

Part II Basic Properties of Viruses and Virus-cell Interaction 65

Chapter 5 Virus Structure and Classification 67

Chapter 6 The Beginning and End of the Virus Replication Cycle 85

Chapter 7 The Innate Immune Response: Early Defense Against Pathogens 105

Chapter 8 Strategies to Protect Against and Combat Viral Infection 131

Part III Working with Virus 153

Chapter 9 Visualization and Enumeration of Virus Particles 155

Chapter 10 Replicating and Measuring Biological Activity of Viruses 163

Chapter 11 Physical and Chemical Manipulation of the Structural Components of Viruses 181

Chapter 12 Characterization of Viral Products Expressed in the Infected Cell 197

Chapter 13 Viruses Use Cellular Processes to Express their Genetic Information 217

Part IV Replication Patterns of Specific Viruses 245

Chapter 14 Replication of Positive-Sense RNA Viruses 247

Chapter 15 Replication Strategies of RNA Viruses Requiring RNA-directed mRNA Transcription as the First Step in Viral Gene Expression 277

Chapter 16 Replication Strategies of Small and Medium-sized DNA Viruses 307

Chapter 17 Replication of Some Nuclear-replicating Eukaryotic DNA Viruses with Large Genomes 335

Chapter 18 Replication of Cytoplasmic DNA Viruses and "Large" Bacteriophages 363

Chapter 19 Retroviruses: Converting RNA to DNA 385

Chapter 20 Human Immunodeficiency Virus Type 1 (HIV-1) and Related Lentiviruses 403

Chapter 21 Hepadnaviruses: Variations on the Retrovirus Theme 415

Part V Molecular Genetics of Viruses 437

Chapter 22 The Molecular Genetics of Viruses 439

Chapter 23 Molecular Pathogenesis 467

Chapter 24 Viral Bioinformatics 477

Chapter 25 Viruses and the Future - Problems and Promises 489

Appendix - Resource Center 503

Technical Glossary 509

Index 533