The Nuclear Membrane

Lipidomic profiling of isolated nuclei from mice and cultured cells.- Profiling plant nuclear envelope composition using subtractive proteomics.- Ultrastructural visualization of the nuclearenvelope in HeLa cells.- Imaging nuclear envelopes using correlative AFM/fluorescence microscopy.- Fast super-resolution live cell imaging of nuclear membrane-endoplasmic reticulum dynamics Immunostaining the yeast nuclear membrane for imaging by super-resolution fluorescence microscopy.- Visualizing phosphatidic acid and diacylglycerol at the nuclear envelope in fission yeast.- Measuring molecular mass densities at subcellular resolution using optical diffraction tomography.- Quantification and comparison of protein distribution on nuclear membrane.- Quantifying nuclear shape fluctuations along the cell cycle, with a focus on early mitosis.- Micropost arrays to model ECM fiber obstacles during cell migration in confinement.- Use of nucleoporin-conjugated beads to study the nuclear pore complex assembly on the nuclear membrane.- T4 DNA-induced reconstruction of artificial nuclei in living mouse oocyte.- Preparation of nucleoplasmic extract and its application in DNA end processing.- Liposome floatation assays to study membrane interactions of nucleoporins.- Giant unilamellar vesicles (GUVs) to study membrane interaction of nucleoporins.- One-pot reconstitution of GPCRs into unilamellar vesicles for fluorescence-based phospholipid scramblase activity assay.- Identifying genomic DNA sequences near the nuclear lamina using proximity biotinylation with Ascorbate Peroxidase.- Pairing LaminB1-DamID and Immuno-3D-FISH to resolve and verify peripheral genome organization of adipogenesis.- Measurement of spatial contact map using sequential FISH.