Zygotic Genome Activation

Probing the genomic distribution of histone modifications in egg extract treated sperm nuclei.- Canonical and Variant Nucleosome Reprogramming from Sperm to Blastula.- Chromatin analyses in mouse oocytes and preimplantation embryos.- Hi-C protocol for studying chromatin spatial organization in early zebrafish development.- HiChIP to Study 3D Genome Organization in Xenopus tropicalis Embryos.- CUT&Tag for transcription factor binding profiles during mammalian zygotic genome activation.- Imaging nuclear clusters in live zebrafish embryos.- Quantifying transcription factor enrichment at target loci in live embryos.- Quantifying Nascent Transcription in Early Embryogenesis.- Ribosomal RNA depletion for poly(A)-tail-independent quantification of genome activation.- Monitoring miRNA expression and activity in mammalian oocytes and early embryos.- Ribo-ITP: A method for ribosome profiling in single cells and low input samples.- Imaging Translation in Early Embryo Development.- Image segmentation software for nuclear segmentation and cell cycle tracking from 4D embryo light sheet data.- Quantitative Staging of Mid-blastula Zebrafish Embryos by Nuclei Counting.