Pichia pastoris

A Brief History of the Pichia pastoris Expression System.- Pichia pastoris Genomic Resources: Accessibility, Recommendations, and Pitfalls.- Isolation of Pichia pastoris Genomic DNA for Long-Read DNA Sequencing and PCR Applications.- Pichia pastoris RNA-Sequencing and Data Analysis.- Localization of Proteins in the Secretory Pathway of Pichia pastoris using Fluorescence Microscopy.- Auxin-Induced Degradation of Target Proteins in Pichia pastoris.- Establishing Reference Genes of Pichia pastoris Quantitative Real-Time Polymerase Chain Reaction Analyses.- ID-MS Based Quantitative Analysis of Metabolites in Pichia pastoris: A Step-By-Step Protocol.- High Throughput Stationary ¹³C-Based Metabolic Flux Analysis of Pichia pastoris Strains.- Determination of Biomass Elemental Composition of Pichia pastoris.- Pichia pastoris Expression Plasmid Assembly using Type IIS Restriction Enzymes.- Autonomously Replicating Sequence-Bearing Plasmids Utilized in Pichia pastoris.- Overlap Extension Construction and Direct Cloning of Loop-Out Prone DNA Expression Cassettes in Pichia pastoris: 75k-?-Secalin from Secale cereale (rye) as a Case Study.- In Vivo Plasmid Assembly in NHEJ-Deficient Pichia pastoris Strains.- Protein Targeting Signals and Strategies for Pichia pastoris.- Cloning of MFa Secretion Variants for Protein Secretion in Pichia pastoris.- Lytic Polysccharide Monooxygenases as Reporters for Signal Peptide Evaluation in Pichia Pastoris.- Exploting Self-Processing 2A Peptides for Multi-Gene Expression in Pichia pastoris.- Retrofitting of AOX1 Promoter-Based Pichia pastoris Expression Strains for Methanol-Independent Protein Production.- Selective Cultivation of Methylotrophic Pichia pastoris Laboratory and Natural Strains.- Transformation of Pichia pastoris by Electroportation.- High Throughput Transformation of Chemical Competent Pichia pastoris Cells using Lithium Acetate/Chloride and Carrier DNA.- High-Efficiency Transformation of Integrative Libraries under Zeocin Selection in Pichia pastoris.- High-Throughput Generation of Pichia pastoris Knock-Out Strains by using CRISPR/Cas9.- Engineering Pichia pastoris Strains using CRISPR/Cas9 Technologies: The Basic Protocol.- Adaptive Laboratory of Pichia pastoris by Serial Batch Cultivation.- Classical Genetics of Pichia pastoris.- Pichia pastoris Random Mutagenesis and Screening.- Cryo-Preservation of Pichia pastoris Strains for Long-Term Storage.- Adapting Pichia pastoris Cultivation Media to REACH Regulations.- Fed-Batch Operational Strategies for Efficient and Robust Pichia pastoris Cultivations Achieving High Cell Densities.- A Guide to Fed-Batch Cultivation of Pichia pastoris on Laboratory Scale.- Pichia pastoris Continuous Cultivations for Strain Characterization and Continuous Biomanufacturing.- Best Practices for Protein Expression in Pichia pastoris Combining Design of Experiments with an Automated Platform for Parallelized Cultivation in Small-Scale Stirred Tank Bioreactors.- Kinetic Modeling of Pichia pastoris Bioreactor Systems under Different Operating Conditions: Batch, Fed-Batch, and Chemostat.- On-Line Monitoring and Control of Methanol Concentration in Pichia pastoris Fermentations.- Off-Gas Balancing for Bioprocess Monitoring Pichia pastoris Bioprocesses.- A Single-Use Technology Platform: How to Set Up Bioprocesses with Pichia pastoris.- A Pichia pastoris Cell-Free Protein Synthesis Platform.- Clarification of Pichia pastoris Broth by TFF with Ceramic Membranes.- Pichia pastoris Cell Disruption by High-Pressure Homogenization (HPH).- Concentration and Purification of Proteins Produced by Pichia pastoris by TFF with Ceramic Membranes.- Protein Purification Protocols for Recombinant Enzymes Produced in Pichia pastoris.- Characterization of Low Molecular Weight Variants (Fragmentation) in Pichia pastoris by CE-SDS.