Duchenne Muscular Dystrophy

Multiplex Ligation-Dependent Probe Amplification (MLPA) for the Detection of Copy Number Mutations in the DMD Gene.- Total RNA-seq as a Tool to Study DMD Splicing and Transcriptional Dynamics.- Preparation, Sectioning, and Histochemical Staining of Muscle Tissue.- Manual Immunofluorescence Staining for Dystrophin and Key Dystrophin-Associated Proteins.- Automated Quantification of Dystrophin Expression by Immunofluorescence in Humans and Animal Models.- Quantitative Evaluation of Dystrophin Expression Using SDS-PAGE Western Blot Methods.- Analysis of Dystrophin-Associated Glycoproteins: Focus on Expression and Glycosylation of Dystroglycan in Skeletal Muscle.- Quantification of Dystrophin Expression by Capillary Immunoassay.- Quantification of Dystrophin in Human Muscle Biopsies by Mass Spectrometry.- Isolation and Culture of Primary Myoblasts from Humans and Mice.- Isolation of Immune Cells from Skeletal Muscles for Flow Cytometry.- Exploring Therapies for Duchenne Muscular Dystrophy using Transdifferentiated Patient Fibroblasts.- Culture and Electrophysiological Analysis of Patient-Specific iPSCs using Microelectrode Array Dishes.- Canine Model of Duchenne Muscular Dystrophy.- In Vivo Electroporation of Plasmid DNA into the Skeletal Muscle of Dystrophic Mouse Models.- Intramuscular and Intravenous AAV-Mediated Gene Delivery in Mouse Models.- Assessment of Contractile and Kinetic Properties of Skeletal and Cardiac Multicellular Preparations in Mouse Models: A Comprehensive Methodological Guide.