Enzymes in RNA Science and Biotechnology Part B

Post-transcriptional modification enzymes1. Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNALi-Sheng Zhang2. Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNALi-Sheng Zhang3. Discovering RNA modification enzymes using a comparative genomics approachSatoshi Kimura4. Functional analysis of tRNA modification enzymes using mutational profilingRyota Yamagami and Hiroyuki Hori5. Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteinsUte Kothe and Sarah Schultz6. Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing AnalysisHoward Gamper, Caroline McCormick, Amr Makhamreh, Meni Wanunu, Sara Rouhanifard and Ya-Ming HouNucleases7. Characterizing RNase L-mediated mRNA decay in single cellsJames Burke, Renee Cusic and Monty Watkins8. Characterization of RNase JBalasubramanian Gopal, Muralidharan Vandanashree and Ankur Singh9. Pri-miRNA cleavage assays for the Microprocessor complexTuan Anh Nguyen, Cong Truc Le and Thi Nhu Y. Le10. The pre-miRNA cleavage assays for DICERTuan Anh Nguyen, Cong Truc Le and Thi Nhu Y. Le11. Methods for study of ribonuclease targeting chimerasMatt Disney, Noah Springer, Samatha Meyer, Yuquan Tong, Jessica Childs-Disney and Raphael Isaac Benhamou12. Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureusWinton Wu, Chi Nam Ignatius Pang, Jai Tree and Daniel Giuseppe Mediati