Biotechnology of Microbial Enzymes: Production, Biocatalysis, and Industrial Applications, Second Edition provides a complete survey of the latest innovations on microbial enzymes, highlighting biotechnological advances in their production and purification along with information on successful applications as biocatalysts in several chemical and industrial processes under mild and green conditions.
The application of recombinant DNA technology within industrial fermentation and the production of enzymes over the last three decades have produced a host of useful chemical and biochemical substances. The power of these technologies results in novel transformations, better enzymes, a wide variety of applications, and the unprecedented development of biocatalysts through the ongoing integration of molecular biology methodology, all of which is covered insightfully and in-depth within the book.
This fully revised, second edition is updated to address the latest research developments and applications in the field, from microbial enzymes recently applied in drug discovery to penicillin biosynthetic enzymes and penicillin acylase, xylose reductase, and microbial enzymes used in antitubercular drug design. Across the chapters, the use of microbial enzymes in sustainable development and production processes is fully considered, with recent successes and ongoing challenges highlighted.
- Explores advances in microbial enzymes from basic science through application in multiple industry sectors
- Includes up-to-date discussions of metabolic pathway engineering, metagenomic screening, microbial genomes, extremophiles, rational design, directed evolution, and more
- Provides a holistic approach to the research of microbial enzymes and their use in sustainable processes and innovation
- Features all new chapters discussing microbial enzyme classes of growing interest, as well as enzymes recently applied in drug discovery and other applications
Auflage
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Techn.
Dateigröße
ISBN-13
978-0-443-19060-5 (9780443190605)
Schweitzer Klassifikation
1. Biotechnology of microbial enzymes: production, biocatalysis, and industrial applications-an overviewGoutam Brahmachari
1.1 Introduction 1.2 An overview of the book 1.2.1 Chapter 2 1.2.2 Chapter 3 1.2.3 Chapter 4 1.2.4 Chapter 5 1.2.5 Chapter 6 1.2.6 Chapter 7 1.2.7 Chapter 8 1.2.8 Chapter 9 1.2.9 Chapter 10 1.2.10 Chapter 11 1.2.11 Chapter 12 1.2.12 Chapter 13 1.2.13 Chapter 14 1.2.14 Chapter 15 1.2.15 Chapter 16 1.2.16 Chapter 17 1.2.17 Chapter 18 1.2.18 Chapter 19 1.2.19 Chapter 20 1.2.20 Chapter 21 1.2.21 Chapter 22 1.2.22 Chapter 23 1.2.23 Chapter 24 1.2.24 Chapter 25 1.2.25 Chapter 261.3 Concluding remarks
2. Useful microbial enzymes-an introductionBeatriz Ruiz-Villafän, Romina Rodri¿guez-Sanoja and Sergio Sänchez
2.1 The enzymes: a class of useful biomolecules 2.2 Microbial enzymes for industry 2.3 Improvement of enzymes 2.4 Discovery of new enzymes 2.5 Concluding remarks Acknowledgments Abbreviations References
3. Production, purification, and application of microbial enzymes Anil Kumar Patel, Cheng-Di Dong, Chiu-Wen Chen, Ashok Pandey and Reeta Rani Singhania
3.1 Introduction 3.2 Production of microbial enzymes 3.2.1 Enzyme production in industries 3.2.2 Industrial enzyme production technology 3.3 Strain improvements 3.3.1 Mutation 3.3.2 Recombinant DNA technology 3.3.3 Clustered regularly interspaced short palindromic repeats-Cas9 technology 3.3.4 Protein engineering 3.4 Downstream processing/enzyme purification 3.5 Product formulations 3.6 Global enzyme market scenarios 3.7 Industrial applications of enzymes 3.7.1 Food industry 3.7.2 Textile industry 3.7.3 Detergent industry 3.7.4 Pulp and paper industry 3.7.5 Animal feed industry 3.7.6 Leather industry 3.7.7 Biofuel from biomass 3.7.8 Enzyme applications in the chemistry and pharma sectors 3.8 Concluding remarks Abbreviations References
4. Solid-state fermentation for the production of microbial cellulasesSudhanshu S. Behera, Ankush Kerketta and Ramesh C. Ray
4.1 Introduction 4.2 Solid-state fermentation 4.2.1 Comparative aspects of solid-state and submerged fermentations 4.2.2 Cellulase-producing microorganisms in solid-state fermentation 4.2.3 Extraction of microbial cellulase in solid-state fermentation 4.2.4 Measurement of cellulase activity in solid-state fermentation 4.3 Lignocellulosic residues/wastes as solid substrates in solid-state fermentation 4.4 Pretreatment of agricultural residues 4.4.1 Physical pretreatments 4.4.2 Physiochemical pretreatment 4.4.3 Chemical pretreatments 4.4.4 Biological pretreatment 4.5 Environmental factors affecting microbial cellulase production in solid-state fermentation 4.5.1 Water activity/moisture content 4.5.2 Temperature 4.5.3 Mass transfer processes: aeration and nutrient diffusion 4.5.4 Substrate particle size 4.5.5 Other factors 4.6 Strategies to improve production of microbial cellulase 4.6.1 Metabolic engineering and strain improvement 4.6.2 Recombinant strategy (heterologous cellulase expression) 4.6.3 Mixed-culture (coculture) systems 4.7 Fermenter (bioreactor) design for cellulase production in solid-state fermentation 4.7.1 Tray bioreactor 4.7.2 Packed bed reactor 4.7.3 Rotary drum bioreactor 4.7.4 Fluidized bed reactor 4.8 Biomass conversions and application of microbial cellulase 4.8.1 Textile industry 4.8.2 Laundry and detergent 4.8.3 Paper and pulp industry 4.8.4 Bioethanol and biofuel production 4.8.5 Food industry 4.8.6 Agriculture 4.9 Concluding remarks Abbreviations References
5. Hyperthermophilic subtilisin-like proteases from Thermococcus kodakarensisRyo Uehara, Hiroshi Amesaka, Yuichi Koga, Kazufumi Takano, Shigenori Kanaya and Shun-ichi Tanaka
5.1 Introduction 5.
