Basic Biotechniques for Bioprocess and Bioentrepreneurship

Part I: Isolation, screening and culture maintenance1. Isolation of microorganisms2. Screening strategies3. Identification, morphological, biochemical, and genetic characterization of microorganisms4. Microbial activity and productivity enhancement strategies5. Culture maintenance, preservation, and strain improvement Part II: Laboratory techniques and instrumentation6. Biomolecules: Types, homogenization, bead beater, sonication7. Centrifugation: Basic principle, types8. Spectroscopy-Principle, types, and applications9. Protein purification: Basic principles and techniques 10. Chromatography: Basic principle, types, and applications11. Electrophoresis: Basic principle, types, and applications Part III: Genomic and proteomic analysis12. DNA, RNA isolation, primer designing, sequence submission, and phylogenetic analysis13. RDT and genetic engineering: Basic of RDT method, PCR, and application14. Protein sequence analysis15. Computational strategies and tools for protein tertiary structure prediction16. Docking strategies 17. A beginner's guide to measuring binding affinity during biomolecular interactions Part IV: Industrially important enzymes18. Enzymes and their significance in the industrial bioprocesses19. Enzyme kinetics: Industrially important enzymes20. Industrial enzymes: Basic information, assay, and applications Part V: Techniques in process development21. Fundamentals of fermentation technology22. Sterilization in bioprocesses23. Scale-up: Lab to commercial scale24. Bioprocess: Control, management, and biosafety issues25. Demonstration and industrial scale-up26. Downstream processing of biotechnology products27. Waste management and environment Part VI: Feasibility, marketing and biobusiness28. Biobusiness opportunities29. Feasibility analysis and business plan30. Patenting, IPR, and regulatory issues31. Commercialization and technology transfers of bioprocess32. Bioproduct marketing strategies