Flow Cytometry in Drug Discovery and Development

Preface. Foreword. Acknowledgements. Contributors. Part I Introduction ( Philip Marder and Virginia Litwin ). 1 Introduction to Flow Cytometry ( Elizabeth Raveche, Fatima Abbasi, Yao Yuan, Erica Salerno, Siddha Kasar and Gerald Marti ). 2 Recent Advances in Flow Cytometry: Platforms, Tools and the Challenges for Data Analysis ( Paul J Smith, Roy Edward, Rachel J Errington ). 3 Introduction to Biomarkers ( Ole Vesterqvist and Manjula Reddy ). Part II Flow Cytometry in the Drug Development Process ( Virginia Litwin and Philip Marder ). 4 HTS Flow Cytometry, Small Molecule Discovery, and the NIH Molecular Libraries Initiative ( Larry A. Sklar and Bruce S. Edwards ). 5 A Multiparameter Approach to Cell Cycle Analysis as a Standard Tool in Oncology Drug Discovery ( Carmen Raventos-Suarez and Byron H. Long ) 6 Flow Cytometry in Pre-Clinical Toxicology/Safety Assessment Environment ( David McFarland and Kristi Harkins ) 7 Use of Flow Cytometry for Defining and Validating the Molecular Mechanism of Anti-cancer Drugs in Humans ( David W Hedley ). 8 CD4 T cell Assessments in Evaluation of HIV Therapeutics ( Thomas N. Denny, Raul Louzao, John Wong and Brooke Walker ). 9 A Translational Medicine Approach to Monitoring the Immune System during Clinical Trials ( Virginia Litwin and James Andahazy ). 10 Immunogenicity Testing Using Flow Cytometry ( Denise M. O'Hara and Valerie Theobald ). 11 Pharmacokinetics by Flow Cytometry: Recommendations for Development and Validation of Flow Cytometric Method for Pharmacokinetic Studies ( Yuanxin Xu and Susan M Richards ). Part III Validation and Regulatory Compliance ( Virginia Litwin and Philip Marder ). 12 Regulatory Compliance and Method Validation ( Carla Hill, Dianna Wu, John Ferbas, Virginia Litwin, Manjula Reddy ). 13 Instrument Validation for Regulated Studies ( John Ferbas and Michelle Schroeder ). Part IV Future Directions ( Virginia Litwin and Philip Marder ). 14 Probability State Modeling: A New Paradigm for Cytometric Analysis ( Bruce Bagwell ). 15 Phospho Flow Cytometry: Single Cell Signaling Networks in Next Generation Drug Discovery and Patient Stratification ( Peter O. Krutzik, Sean C. Bendall, Matthew B. Hale, Jonathan M. Irish, Garry P. Nolan ). Index.