This volume explores plant immunity through protocols for recently established genetic and biochemical techniques to elucidate immune mechanisms mediated by pattern-recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). The book covers the identification of PRRs, their associated downstream immune regulators, PRR-ligand interaction-based immune responses, identification of NLR complex components, pan-NLRome analysis, resistosome assays, and cell death evaluation. Additionally, it presents protocols for co-transcriptome analysis of plant-pathogen interactions, single-nucleus multi-omics, metabolite imaging, and bioluminescence-based quantification of pathogen growth, as well as genome editing tools that contribute to the development of disease-resistant plants. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Plant Immunity: Methods and Protocols
serves as an ideal guide for researchers aiming to accelerate ongoing research and advances in the field of plant immunity.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
50
23 s/w Abbildungen, 50 farbige Abbildungen
VIII, 162 p. 73 illus., 50 illus. in color.
ISBN-13
978-1-0716-5138-4 (9781071651384)
Schweitzer Klassifikation
