Spermatology: Current Methods to Study Sperm Classical Variables.- Evolutionary Aspects of Sperm Physiology and Its Assessment.- Assessment of Boar Semen Morphology and Morphometry.- Semen Extraction and Analysis in Domestic Animals.- Semen Extraction and Management in Wild Animals: TUMASG Technique.- Semen Extraction and Analysis in Avian Species.- Boar Semen Assessment for the Production of Artificial Insemination Doses.- Semen Evaluation in the Production of Bovine Artificial Insemination Doses.- Sperm Vitrification in Horse and Donkey.- Cryopreservation of Dog Semen.- Rabbit Sperm Cryopreservation.- Cryopreservation of Small Ruminant Sperm and Detection of Sperm Cryocapacitation.- Sperm Cyropreservation in Boars.- Cryopreservation of Horse Sperm.- New Delivery Systems on Sperm Quality Improvement.- Assessment of Sperm Motility with the Use of Computer-Aided Sperm Analysis (CASA).- Bioinformatics on Sperm Subpopulations using Computer Assisted Sperm Analysis (CASA).- Sperm Selection by Colloid Centrifugation.- Bioenergetics of Rodent Spermatozoa.- Flow Cytometry in the Assessment of Sperm Function Parameters: Viability, Acrosomal Integrity, Membrane Stability, and Mitochondrial Status.- Dextran/Swim-Up Procedure for Ram Sperm Selection.- Chromatin Structure Assessment Using Flow Cytometry after DNA Denaturation and Acridine Orange Staining.- Assessment of the Sperm DNA Oxidative Damage by Immunofluorescence and Flow Cytometry.- Sperm Intracellular Calcium Evaluation.- Oxidative Status Measurement in Domestic and Wild Small Ruminants' Species.- Evaluation of Oxidative Stress in Mammalian Spermatozoa.- Real-Time Acrosome Reaction and Intracellular Calcium Recordings in Mice Sperm.- Field Emission Scanning Electron Microscopy (FE-SEM) as an Approach for Membrane Surface Mapping in Cell Biology.- Detection of Sperm Membrane Proteins by Immunocytochemistry, Scanning and Transmission Electron Microscopy, and Western Blotting.- Detection and Quantification of mRNA in Mammalian Spermatozoa.- Techniques to Determine Mammalian Sperm Capacitation.- Disulfide Bridges Assessment in the Sperm Chromatin by Flow Cytometry.- Analysis of the Chromatin Structure by Chromomycin A3 (CMA3) and Flow Cytometry.- Isolation of High-Quality RNA from Mammalian Spermatozoa for Transcriptome Studies.- Prediction of CircRNAs in Mammalian Spermatozoa from RNA Sequencing Data.- Reduced Representation Bisulfite Sequencing Library Preparation for Sperm DNA Methylation Analyses: Manuel and Automated Methods.- Production of Healthy Mice from Freeze-Dried Spermatozoa Preserved at Room Temperature.- High Throughput Analysis of Post-Meiotic Male Germ Cells Using Image Flow Cytometry.- Assessment of Bacterial Contamination in Semen.- A Size-Exclusion Chromatography-Based Procedure for Isolating Extracellular Vesicle Subsets from Porcine Semina Plasma.- Molecular Determinants in Pig Semen: Verified Protocols for Cytokine Detection and Quantification.- Molecular Determinants in Semina Plasma and Spermatozoa: Non-Targeted Metabolomics.- Seminal Plasma Proteomics using Filter Aided Sample Preparation.- Assessing Seminal Fluid Interaction with Female Reproductive Tract Tissues: In Vivo and In Vitro Models for Mouse.- Seminal Fluid Interaction with Female Reproductive Tract Epithelial Cells: In Vitro Models for Human.- Impact of Seminal Plasma on the Embryonic Development and Uterine Environment in Pigs.- Assessment of Sperm Binding Capacity in the Reservoir using an In Vitro Model of Oviduct Epithelial Spheroids in Cattle.- Characterization of Ovine Cervical Mucus and Implementation of a Sperm-Mucus Penetration Test Using Fluorescently Labeled Sperm.
