Plant MicroRNAs

The Use of Arabidopsis thaliana to Characterize the Production and Action Stages of the Plant MicroRNA Pathway.- An overview of miRNA and miRNA target analysis tools.- Prediction of miRNA targets in plants.- An overview of miRNA and miRNA target analysis tools.- Scalable Approach to Evaluate Plant microRNA Trimming and Tailing from Small RNA-Seq data.- In vitro transcription of plant miRNA for structural and processing analysis.- Cost-Effective and Sensitive Probe-Based Universal (PBU) qPCR for MicroRNA Quantification in Plants.- Northern blot analysis of plant microRNAs and other small RNAs.- Northern blot analysis of plant microRNAs and other small RNAs.- Determination of expression patterns of plant miRNAs by Transcriptional Fusion using GUS and Fluorescent reporters.- A Visual Reporter System for Analyzing Small RNA-Triggered Local and Systemic Silencing of an Endogenous Plant Gene.- Unravelling tissue specific miRNAs role in plants exploiting the GAL4/UAS system. -The Luciferase reporter on intronic microRNA-directed regulation.- A simple and efficient protocol to transform and regenerate CRISPR-Cas9-mediated genome edited tomato plants.- CRISPR-activation: boosting expression of plant MIRs.- Gene silencing in plants through exogenous application of miRNAs.- A strategy to use Artificial MicroRNAs for Gene silencing and Overcome (AmiGO).- Degradome sequencing.- Whole mount staining and immunolocalization procedures for developing ovules of Vigna unguiculata.- Bioinformatic enrichment and network analyses of miRNA-related high-throughput sequencing data.- Identifying cis-regulatory binding sites in promoter regions of miRNA genes: A step-by-step protocol.- A Detailed Protocol for the Isolation of Arabidopsis thaliana Mesophyll Protoplasts and their Applications.- A protocol for live imaging of Arabidopsis gynoecium development using confocal microscopy.