Visible and Near Infrared Absorption Spectra of Human and Animal Haemoglobin determination and application

Foreword -- Preface -- Chapter 1: IntroductIon -- Visual spectrophotometry -- Photoelectric spectrophotometry -- Diode array spectrophotometry -- Biomedical applications -- Chapter 2: Ddinitions and terminology -- Spectrophotoinetry -- Haemoglobin and haemoglobin derivatives -- Oxygen carrying propeilies of mammalian blood -- Chapter 3: Spectrophotometry -- The laws of absorption of radiant energy -- Applications of Lambert-Beer's law -- Instrumentation -- Spectrophotometer performance -- Chapter 4: Total haemoglobin concentration -- Chapter 5: Absorptlvfty at 540 nm of haemiglobincyanide -- Preparation of a haemoglobin stock solution -- Determination of the iron concentration of the haemoglobin stock solution -- Determination of the absorbance at 540 nm of the haemoglobin stock solution after conversion to HiCN -- Calculation of the absorptivny at 540 nm of HICN -- e(54O) as found for human and animal haemoglobin -- Chapter 6: PreparatIon of haemoglobln derivatives -- Specimen procurement and handling -- Tonometry, eiythmlysis and filtration -- Preparation of HHb, O2Hb, and COHb -- Preparation of Hi, HiCN. and SHb -- Chapter 7: Determination of absorption spectra -- Lightpath length -- Absorbance measurement -- Measurement of total haemoglobin -- Measurement of loetal haemoglobin -- Absorptivity calculation -- Influence of temperature and pH -- Chapter 8: Absorption spectra of human IlbA and HbF -- Absoiptivity of HbA: standard procedure -- Absoqtivity of HbA: SFH procedure -- Absorptivity of HbF: standard procedure -- Comments -- Spectral chanictenstics of modified human haemoglobin -- Chapter 9: Absorption spectra of dog haemoglobin -- Chapter 1O Absorption spectra of rat hwnoglobin -- Chapter 11: Absorptio, spectra of bovrne haemoglobin -- Chapter 12: Absorption spectra of pig haem.oglobin -- Chapter 13: Absorption spectra of horse haemoglobin -- Chapter 14: Absorption spectra of sheep haemoglobin -- Absorptiviiy of adult haemoglobm -- Absorptivlty of foetal haemoglobin -- Comments -- Chapter 15: Comments on the determination of absorption spectra of haemoglobia -- Chapter 16: Haemoglobinometry -- A shoct history -- The reference point of standardised haemoglobinome*iy -- The haemiglobincyanide method -- Haemiglobincyanide standards -- Routine haemoglobinometry -- Chapter 17: Muftlconiponent analysis of haemoglobln dedvadves -- The lengthy road to MCA -- Selected twowaveleagth methods for 021Th and COHb -- Multicoinponent analysis -- Multiwavelength haemoglobin photometers -- MCA including Hi and HICN -- Current developments -- Chapter 18: Oxlmetry and related techniques -- Development of oximetry -- Cuvette oximetiy and densitometry -- Syllectometry -- Fibre optic oximetry -- Pulse oximetry -- Near infrared spectroscopy -- Chapter 19: The oxygen binding capacity of human haemoglobin -- Early determinations of the oxygen binding capacity of human haemoglobin -- Reference method for total oxygen in blood -- Redetermination of the oxygen binding capacity of human haemoglobin -- Routine determination of the oxygen capacity of human blood -- Chapter 20: The oxygen affinity of human hacmoglobin -- Instniments and procedures -- The normal oxygen dissociation curve of human blood -- Effect of temperature -- The Bohr and Haldane effects -- Effect of 2,3dipliosphoglycerate -- Effect of dyshaemoglobins -- Conclusions -- References -- Abbreviations and symbols -- Index.