This book provides state-of-the-art information on gene essentiality screenings in a wide variety of organisms, i.e. screening for protein-coding genes and other genomic elements that are required by an organism to survive under specific conditions. With a focus on the two techniques that have revolutionized the field, the collection begins with chapters employing CRISPR/Cas9-based approaches followed by Tn-seq-based approaches, but later chapters also delve into other techniques for exploring essential genes, such as bioinformatics methods. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative,
Essential Genes and Genomes: Methods and Protocols
is an ideal guide for researchers attempting to strip genetics down to its fundamentals.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
50
18 s/w Abbildungen, 50 farbige Abbildungen
XII, 434 p. 68 illus., 50 illus. in color.
Dateigröße
ISBN-13
978-1-0716-1720-5 (9781071617205)
DOI
10.1007/978-1-0716-1720-5
Schweitzer Klassifikation
A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9.- CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell Lines.- Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes.- Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen
Candida albicans
.- Identification of Essential Genes Using Sequential CRISPR and siRNA Screens.- A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines.- New Method for Genome-Scale Functional Genomics Study in Bacteria with Superior Performance: CRISPR Interference Screen.- A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria.- A Method of Transposon Insertion Sequencing in Comprehensively Identifying
Vibrio vulnificus
Genes Required for Growth in Human Serum.- The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of
Pseudomonas aeruginosa
.- Analysis of
Escherichia coli
K1 Virulence Genes by Transposon-Directed Sequencing.- Using Genome Scale Mutant Libraries to Identify Essential Genes.- Identification and Analysis of Essential Genes in
Streptococcus mutans
with Transposon Sequencing.- The Use of TnSeq to Identify Essential Alphaproteobacterial Genes Reveals Operational Variability in Conserved Developmental and Cell Cycle Systems.- A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study
of Bifidobacterium breve
UCC2003 as a Prototype of Its Genus.- The Application of Transposon Insertion Sequencing in Identifying Essential Genes in
B. fragilis
.- Repressible Promoter System to Study Essential Genes in Mycobacteria.- A Single-Cell RNA-Seq-Based Approach for Genome-Wide Identification ofCell Essential Genes.- Identification of Essential Genes in
Caenorhabditis elegans
with Lethal Mutations Maintained by Genetic Balancers.- A Screening Method to Identify Essential Yeast Genes for Responses Towards Spermine.- An Effective Pre-Processing Method for High-Quality Pan-Genome Analysis of
Bacillus subtilis
and
Escherichia coli
.- Analysis of Gene Essentiality from TnSeq Data Using Transit.- Geptop 2.0: Accurately Select Essential Genes from the List of Protein-Coding Genes in Prokaryotic Genomes.
