An Introduction to Molecular Biotechnology

FUNDAMENTALS OF CELLULAR AND MOLECULAR BIOLOGY
The Cell as the Basic Unit of Life
Structure and Function of Cellular Macromolecules
Structure and Functions of a Cell
Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins)
Distributing Proteins in the Cell (Protein Sorting)
Evolution and Diversity of Organisms

STANDARD METHODS IN MOLECULAR BIOTECHNOLOGY
Isolation and Purification of Proteins
Peptide and Protein Analysis with Electrospray Tandem Mass Spectrometry
Isolation of DNA and RNA
Chromatography and Electrophoresis of Nucleic Acids
Hybridization of Nucleic Acids
Use of Enzymes in the Modification of Nucleic Acids
Polymerase Chain Reaction
DNA Sequencing
Genome Editing
Cloning Procedures
Expression of Recombinant Proteins
Omics technologies
Patch Clamp Method
Cell Cycle Analysis
Microscopic Techniques
Laser Applications

KEY TOPICS
Genomics and Functional Genomics
Bioinformatics
Cellular Systems Biology
Protein-Protein and Protein-DNA Interaction
Drug Research
Drug Targeting and Prodrugs
Molecular Diagnostics and Personalized Medicine
Recombinant Antibodies and Phage Display
Transgenic and Gene-Targeted Mice
Plant Biotechnology
Industrial Biocatalysis

BIOTECHNOLOGY IN INDUSTRY
The Biotech Industry, Markets, and Opportunities
Patents in the Molecular Biotechnology Industry: Legal and Ethical Issues
Drug Approval in the European Union and United States
Founding a Biotech Company

APPENDIX
Further Reading
Abbreviations
Subject Index