Mitochondrial Translocases Part A

1. Isolation of yeast mitochondria by differential centrifugationHeike Rampelt2. Isolation of yeast mitochondria by affinity purification using magnetic beadsPin-Chao Liao, Liza Pon, Tzu-Ying Lin and Shih-Hung Chien3. A modified procedure for the separation of mitochondria from peroxisomes Doron Rapaport and Nitya Aravindan4. Subcellular fractionation for the analysis of mitochondrial proteinsNils Wiedemann and Conny Steiert5. Monitoring mitochondrial localization of dual localized proteins using a Bi-Genomic Mitochondrial-Split-GFPHubert Dominique Becker6. Analysis of mitochondrial localization and biogenesis by genetic screens and automated imagingMaya Schuldiner and Yury S. Bykov7. Methods to monitor mitochondrial disulfide bondsAgnieszka Chacinska, Ben Hur Mussulini, Michal Wasilewski and Klaudia K Maruszczak8. Prediction of mitochondrial targeting signals and their cleavage sitesPaul Horton, Kenichiro Imai and Yoshinori Fukasawa9. Monitoring of mitochondrial preprotein processing, peptide accumulation and degradationNora Vögtle and Cansu Kücükköse10. Analysis of mitochondrial targeting signal cleavage and processing by mass spectrometryPitter F. Huesgen11. Analysis of targeting signals for mitochondrial intermembrane space importKostas Tokatlidis12. Analysis and prediction of internal mitochondrial targeting signalsTimo Mühlhaus, Johannes M. Herrmann, Saskia Rödl and Felix Jung13. Monitoring and analysis of mitochondrial precursor protein aggregates in the cytosolAgnieszka Chacinska14. In vitro translation of mitochondrial precursor proteins in yeast extract to study interactions with cytosolic chaperonesLayla Drwesh15. In vitro import of mitochondrial precursor proteins into yeast mitochondriaDejana Mokranjac16. Monitoring the import and assembly of mitochondrial precursor proteins into human cell line mitochondriaDiana Stojanovski and Jordan Crameri17. Import of mitochondrial precursor proteins into mitochondria with semi- intact cellsJohannes M. Herrmann18. The MitoLuc Assay for the Analysis of the Mechanism of Mitochondrial Protein ImportIan Collinson, Hope Isobel Needs, Youmian Yan and Natalie Niemi19. Monitoring mitochondrial protein import by live cell imagingFX Reymond Sutandy20. Quantification of mitochondrial protein import by mass spectrometryChristian Münch21. Methods to analyze mitochondrial protein translocation in plant mitochondriaMonika W. Murcha22. In organello silencing of mitochondrial gene expressionLuis Daniel Cruz-Zaragoza and Mats Koschel23. Assays to monitor mitochondrial translationMartin Ott24. Analysis of mitochondrial translation using click chemistrySven Dennerlein and Roya Yousefi