The principles of fluorescence spectroscopy are by now well established, and, after a rather lengthy gestation period, the technique is now routinely applied to a broad spectrum of problems, ranging from mechanistic photo chemistry to chemical analyses in biomedical and environmental systems of structure and function in biological macromolecules. Phosphor to probes escence spectrometry and chemiluminescence are also well-known tech niques; they are somewhat less well established than fluorescence (at least in analytical chemistry), but they too are receiving greatly increased appli cation to both laboratory and "real" problems. This is not to imply that luminescence spectroscopy, viewed in its broadest sense, is a static field. In fact, recent advances in instrumentation make it feasible to apply fluorescence to problem areas in which its use five years ago would have been unthinkable. Advances in hardware generate advances in application, and very significant progress is being recorded in the application of fluorescence (and its close relatives, phosphorescence and chemiluminescence) in the biochemical, biomedical, and environmental spheres.
Reihe
Auflage
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
ISBN-13
978-1-4684-2583-3 (9781468425833)
DOI
10.1007/978-1-4684-2583-3
Schweitzer Klassifikation
of Volume 1.- 1 Fluorescence Spectroscopy with On-Line Computers: Methods and Instrumentation.- A. Introduction.- B. A Simple On-Line Data-Acquisition and Control System.- C. Integrated-Computerized Instrument Systems for Fluorescence Spectroscopy.- D. Glossary.- References.- 2 Digital and Analog Measurements in Fluorescence Spectroscopy.- A. Introduction.- B. Definitions and Nomenclature.- C. Comparison of Electronic Methodology in Two Modern Spectrofluorometers.- D. Photon Counting and Lock-in Amplification.- E. Principles and Applications of Digital Integration.- F. Other Electronic Techniques for Fluorometry.- References.- 3 Modulation and Derivative Techniques in Luminescence Spectroscopy: Approaches to Increased Analytical Selectivity.- A. Introduction.- B. Derivative Techniques in Luminescence Spectrometry.- C. Wavelength-Modulation Techniques in Luminescence Spectrometry.- D. Conclusion.- References.- 4 Dye Lasers: Fundamentals and Analytical Applications.- A. Introduction.- B. Instrumentation.- C. Analytical Applications.- D. Conclusion.- References.- 5 Laser-Excited Fluorescence Techniques in Air Pollution Monitoring.- A. Introduction.- B. Measurement of Atmospheric NO2 Levels.- C. Fluorescence of Atmospheric Aerosols.- D. Fluorescence Determination of SO2.- E. Assessment of the Fluorescence Method.- F. Application of Novel Laser Techniques.- G. Conclusions and Future Projections.- References.- 6 Chemiluminescence Techniques in Air Pollutant Monitoring.- A. Introduction.- B. Monitors in Present Use.- C. Compound-Specific Monitoring Methods in Development or under Consideration.- D. Conclusion.- Appendix: Pertinent Rate Coefficients.- References.- 7 Chemiluminescence Analysis in Water Pollution Studies.- A. Introduction.- B. ATP Analysis.- C. Iron(II) Analysis.- D. Phosphorus Analysis.- E. Future Developments in Chemiluminescence Analysis.- References.- 8 Internal-Reflection Techniques in Fluorescence Spectroscopy.- A. Introduction.- B. Single-Internal-Reflection Studies.- C. Multiple-Internal-Reflection Fluorescence (MIRF).- D. Optical Materials and Geometries for Internal-Reflection Elements (IRE).- E. MIRF with a Commercial Spectrophotofluorometer.- References.- Author Index.
