Fluorescence is more and more widely used as a tool of investigation, analysis, control and diagnosis in many fields relevant to physical, chemical, biological and medical sciences. New technologies con tinuously emerge thanks to the progress in the design of light sources (e.g. laser diodes), detectors (3D, 4D) and compact ultrafast elec tronic devices. In particular, much progress has been made in time resolved fluorescence microscopy (FUM: Fluorescence Lifetime Imaging Microscopy; FCS: Fluorescence Correlation Spectroscopy). Furthermore, the sensitivity now allows one to detect a single mole cule in the restricted field of a confocal microscope, which actually offers the possibility to study phenomena at a molecular level. The development of new fluorescent probes is still a necessity. In particular, the growing use of lasers implies high resistance to photo degradation. Fluorescence emission at long wavelengths is also a distinct advantage. Furthermore, in vivo inclusion of new fluorescent aromatic residues in proteins offer new potentialities in biology. of ions and molecules is Fluorescence-based selective detection still the object of special attention. Considerable effort is being made in the design of supramolecular systems in which the recognition event is converted into a fluorescence signal easily detected. New fluorescent sensors for clinical diagnosis and detection of pollutants in atmosphere and water are extensively developed. All these developments justify the regular publication of books giving the state-of-the-art of the methods and applications of fluo rescence spectroscopy.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
4
207 s/w Abbildungen, 4 farbige Abbildungen
XXV, 490 p.
Dateigröße
ISBN-13
978-3-642-56853-4 (9783642568534)
DOI
10.1007/978-3-642-56853-4
Schweitzer Klassifikation
1 Historical Aspects of Fluorescence.- 1 Introduction: On the Origin of the Terms Fluorescence, Phosphorescence, and Luminescence.- 2 Pioneering Contributions of Jean and Francis Perrin to Molecular Luminescence.- 3 The Seminal Contributions of Gregorio Weber to Modern Fluorescence Spectroscopy.- 2 Fluorescence of Molecular and Supramolecular Systems.- 4 Investigation of Femtosecond Chemical Reactivity by Means of Fluorescence Up-Conversion.- 5 Spectroscopic Investigations of Intermolecular Interactions in Supercritical Fluids.- 6 Space and Time Resolved Spectroscopy of Two-Dimensional Molecular Assemblies.- 7 From Cyanines to Styryl Bases - Photophysical Properties, Photochemical Mechanisms, and Cation Sensing Abilities of Charged and Neutral Polymethinic Dyes.- 8 Phototunable Metal Cation Binding Ability of Some Fluorescent Macrocydic Ditopic Receptors.- 3 Fluorescence in Sensing Applications.- 9 The Design of Molecular Artificial Sugar Sensing Systems.- 10 PCT (Photoinduced Charge Transfer) Fluorescent Molecular Sensors for Cation Recognition.- 11 Fluorometric Detection of Anion Activity and Temperature Changes.- 12 Oxygen Diffusion in Polymer Films for Luminescence Barometry Applications.- 13 Dual Lifetime Referencing (DLR) - a New Scheme for Converting Fluorescence Intensity into a Frequency- Domain or Time-Domain Information.- 4 New Techniques of Fluorescence Microscopy in Biology.- 14 Two-Photon Fluorescence Fluctuation Spectroscopy.- 15 Fluorescence Lifetime Imaging Microscopy of Signal Transduction Protein Reactions in Cells.- 16 New Techniques for DNA Sequencing Based on Diode Laser Excitation and Time-Resolved Fluorescence Detection.- 17 The Integration of Single Molecule Detection Technologies into Miniaturized Drug Screening: Current Status and FuturePerspectives.- 18 Picosecond Fluorescence Lifetime Imaging Spectroscopy as a New Tool for 3D Structure Determination of Macromolecules in Living Cells.- 5 Proteins and Their Interactions as Studied by Fluorescence Methods.- 19 About the Prediction of Tryptophan Fluorescence Lifetimes and the Analysis of Fluorescence Changes in Multi-Tryptophan Proteins.- 20 Application of Time-Resolved Fluorescence Spectroscopy to Studies of DNA-Protein Interactions and RNA Folding.- 21 Rare Earth Cryptates and TRACE Technology as Tools for Probing Molecular Interactions in Biology.- 22 Tracking Molecular Dynamics of Flavoproteins with Time-Resolved Fluorescence Spectroscopy.
