Genetically Incorporated Non-Canonical Amino Acids

Focused Engineering of Pyrrolysyl-tRNA Synthetase-Based Orthogonal Translation Systems for the Incorporation of Various Non-Canonical Amino Acids.- Engineering Homogeneous Photoactive Antibodies Fragments.- Repurposing Photosensitizer Proteins through Genetic Code Expansion to Facilitate Photo-Biocatalysis.- Genetic Encoding of a Fluorescent Non-Canonical Amino Acid as a FRET Donor for Analysis of Deubiquitinase Activities.- Creating Selenocysteine-Specific Reporters Using Inteins.- Protein Expression with Biosynthesized Non-Canonical Amino Acids.- Reprogramming Initiator and Nonsense Codons to Simultaneously Install Three Distinct Non-Canonical Amino Acids into Proteins in E. coli.- Encoding Non-Canonical Amino Acids into Phage Displayed Proteins.- Genetically Encoded Non-Canonical Amino Acids in Proteins to Investigate Lysine Benzoylation.- Semi-Synthesis of Glutamine-Methylated Proteins Enabled by Genetic Code Expansion.- Genetic Code Expansion in Mammalian Cells.- Generation of Amber Suppression Cell Lines Using CRISPR-Cas9.- Genetic Code Expansion in Mammalian Cells through Quadruplet Codon Decoding.- Genetically Encoded 1,2-Aminothiol for Site-Specific Modification of a Cellular Membrane Protein via TAMM Condensation.- Conformational GPCR BRET Sensors Based on Bioorthogonal Labeling of Non-Canonical Amino Acids.- Selective Inhibition of Kinase Activity in Mammalian Cells by Bioorthogonal Ligand Tethering.- Site-Specific Incorporation of Sulfotyrosine into Proteins in Mammalian Cells.- Small Molecule Phosphine Activation of Protein Function in Zebrafish Embryos with an Expanded Genetic Code.- Non-Canonical Amino Acid Incorporation in Mice.