This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,
Crop Breeding:
Genetic Improvement Methods
aims to ensure successful results in the further study of this vital field.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
54 farbige Abbildungen, 8 s/w Abbildungen
XI, 280 p. 62 illus., 54 illus. in color.
Dateigröße
ISBN-13
978-1-0716-1201-9 (9781071612019)
DOI
10.1007/978-1-0716-1201-9
Schweitzer Klassifikation
