A Brief Introduction into the Renin-Angiotensin-Aldosterone System: New and Old Techniques.- A Color Segmentation Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining.- Assessment of Protein Carbonylation and Protein Tyrosine Phosphatase (PTP) Oxidation in Vascular Smooth Muscle Cells (VSMCs) using Immunoblotting Approaches.- Methods for Studying the Role of RAAS in the Modulation of Vascular Repair-Relevant Functions of Stem/Progenitor Cells.- Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta.- Measuring Blood Pressure using a Non-Invasive Tail Cuff Method in Mice.- Blood Pressure Monitoring using Radio Telemetry Method in Mice.- Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry.- Assessment of the Renin-Angiotensin System in Cellular Organelle: New Arenas for Study in the Mitochondria.- Comprehensive Assessments of Energy Balance in Mice.-
In Vitro
Assays to Determine Smooth Muscle Cell Hypertrophy, Protein Content, and Fibrosis.- A New Mouse Model for Introduction of Aortic Aneurysm by Implantation of Deoxycorticosterone Acetate Pellets or Aldosterone Infusion in the Presence of High Salt.- Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors.- A Primer to Angiotensin Peptide Isolation, Stability, and Analysis by Nano-Liquid Chromatography with Mass Detection.- Analysis of Angiotensin Metabolism in the Kidney using Mass Spectrometry.
