Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods

1. Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA HelicasesT.L. Pavankumar, J.C. Exell and S.C. Kowalczykowski2. Single Molecule Imaging with one Color FluorescenceY. Qiu and S. Myong3. Measuring Membrane Protein Dimerization Equilibrium in Lipid Bilayers by Single-Molecule Fluorescence MicroscopyR. Chadda and J.L. Robertson4. Fluorescent Labeling of Proteins in Whole Cell Extracts for Single-Molecule ImagingS.R. Hansen, M.L. Rodgers and A.A. Hoskins5. Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence MicroscopyE.M. Boehm, S. Subramanyam, M. Ghoneim, M.T. Washington and M. Spies6. How to Measure Separations and Angles Between Intra-Molecular Fluorescent MarkersK.I. Mortensen, J. Sung, J.A. Spudich and H. Flyvbjerg7. Precisely and Accurately Inferring Single-Molecule Rate ConstantsC.D. Kinz-Thompson, N.A. Bailey, R.L. Gonzalez8. Quantification of Functional Dynamics of Membrane Proteins Reconstituted in Nanodiscs Membranes by Single Turnover Functional ReadoutM.E. Moses, P. Hedegård and N.S. Hatzakis9. Putting Humpty-Dumpty Together: Clustering the Functional Dynamics of Single Biomolecular Machines Such as the SpliceosomeC.E. Rohlman, M.R. Blanco and N.G. Walter10. Single Molecule FRET to Measure Conformational Dynamics of DNA Mimatch Repair ProteinsJ.W. Gauer, S. LeBlanc, P. Hao, R. Qiu, B. Case, M. Sakato, M.M. Hingorani, D.A. Erie and K.R. Weninger11. Single Molecule Confocal FRET Microscopy to Dissect Conformational Changes in the Catalytic Cycle of DNA TopoisomerasesS. Hartmann, D. Weidlich and D. Klostermeier12. Probing the Conformational Landscape of DNA Polymerases Using Diffusion-Based Single-Molecule FRETJ. Hohlbein and A.N. Kapanidis13. Methods for Investigating DNA Accessibility with Single NucleosomesM.D. Gibson, M. Brehove, Y. Luo, J. North and M.G. Poirier14. Single-Molecule Fluorescence Studies of Fast Protein FoldingZ. Wang, L.A. Campos and V. Muñoz15. Single-Molecule Multi-Color FRET Assay for Studying Structural Dynamics of BiomoleculesS. Lee, Y. Jang, S.-J. Lee and S. Hohng16. A Multi-Color Single Molecule FRET Approach to Study Protein Dynamics and Interactions SimultaneouslyM. Götz, P. Wortmann, S. Schmid and T. Hugel17. Interferometric Scattering Microscopy for the Study of Molecular MotorsJ. Andrecka, Y. Takagi, K.J. Mickolajczyk, L.G. Lipert, J.R. Sellers, W.O. Hancock, Y.E. Goldman and P. Kukura18. Enzyme Kinetics in Femtoliter ArraysP. Mogalisetti and D.R. Walt