Mechanisms of DNA Recombination and Genome Rearrangements: Methods to Study Homologous Recombination

1. Processing of DNA Double-Strand Breaks in YeastRobert Gnügge, Julyun Oh and Lorraine S. Symington2. Methods to Study DNA End Resection I: Recombinant Protein PurificationRoopesh Anand, Cosimo Pinto and Petr Cejka3. Methods to Study DNA End Resection II: Biochemical Reconstitution AssaysCosimo Pinto, Roopesh Anand and Petr Cejka4. Direct Quantitative Monitoring of Homology-Directed DNA Repair of Damaged TelomeresPriyanka Verma, Robert L. Dilley, Melina T. Gyparaki and Roger A. Greenberg5. Kinetic Analysis of the Exonuclease Activity of the Bacteriophage T4 Mre11-Rad50 ComplexTibebe A. Teklemariam, Osvaldo D. Rivera and Scott W. Nelson6. Observation and Analysis of RAD51 Nucleation Dynamics at Single-Monomer ResolutionShyamal Subramanyam, Colin D. Kinz-Thompson, Ruben L. Gonzalez and Maria Spies7. Determining the RAD51-DNA Nucleoprotein Filament Structure and Function By Cryo-Electron MicroscopyLingyun Zhao, Jingfei Xu, Weixing Zhao, Patrick Sung, and Hong-Wei Wang8. Expression, Purification, and Biochemical Evaluation of Human RAD51 ProteinShyamal Subramanyam and Maria Spies9. TIRF-Based Single-Molecule Detection of the RecA Presynaptic Filament DynamicsSung H. Kim10. The RadA Recombinase and Paralogs of the Hyperthermophilic Archaeon Sulfolobus SolfataricusMichael L. Rolfsmeier and Cynthia A. Haseltine11. Reconstituting the 4-Strand DNA Strand ExchangeOlga M. Mazina and Alexander V. Mazin12. Purification Of Saccharomyces Cerevisiae Homologous Recombination Proteins Dmc1 and Rdh54/Tid1 and a Fluorescent D-Loop AssayYuen-Ling Chan and Douglas K. Bishop13. Probing Dynamic Assembly and Disassembly of Rad51 Tuned by Srs2 Using smFRETYupeng Qiu, Hye R. Koh and Sua Myong14. The Recombination Mediator BRCA2: Architectural Plasticity of Recombination Intermediates Revealed by Single-Molecule Imaging (SFM/TIRF)Arshdeep Sidhu, Dejan Ristic, Humberto Sánchez and Claire Wyman15. Single-Molecule Dynamics and Localization of DNA Repair Proteins in CellsMaarten W. Paul, Alex N. Zelensky, Claire Wyman and Roland Kanaar16. Single-Stranded DNA Curtains for Studying the Srs2 Helicase Using Total Internal Reflection Fluorescence MicroscopyLuisina De Tullio, Kyle Kaniecki and Eric C. Greene17. Single-Molecule Analysis of Replication Protein A-DNA InteractionsFletcher E. Bain, Laura A. Fischer, Ran Chen and Marc S. Wold18. Single-Molecule Studies of ssDNA-Binding Proteins ExchangeOlivia Yang and Taekjip Ha19. Dissecting the Recombination Mediator Activity of BRCA2 Using Biochemical MethodsCatharina von Nicolai, Åsa Ehlén, Juan S. Martinez and Aura Carreira20. Approaches to Understanding the Mediator Function of Brh2 in Ustilago maydisQingwen Zhou, William K. Holloman and Milorad Kojic21. GEN1 Endonuclease: Purification and Nuclease AssaysYing Wai Chan and Stephen C. West22. Biochemical and Structural Properties of Fungal Holliday Junction-Resolving EnzymesYijin Liu, Alasdair Freeman, Anne-Cécile Déclais, Anton Gartner and David M. J. Lilley23. Preparation and Resolution of Holliday Junction DNA Recombination IntermediatesRajvee Shah Punatar and Stephen C. West