One-Step Generation of a Drug-Releasing Microarray for High-Throughput Small-Volume Bioassays

Abstract.- Table of Contents.- List of Tables.- List of Figures.- Chapter 1. Introduction.- 1.1. High-Throughput Small-Volume Bioassays.- 1.2. Developmental Goal for the 'Pipetting-Free' HTS Platforms.- 1.3. Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-Assembly of Drug-Laden Microparticles (DLPs).- Chapter 2. System Development.- 2.1. Sealing-Film Assisted Seeding Method for Saving Cell Consumptions.- 2.2. Chip and Jig Development.- 2.3. Preparation of DLPs Library.- 2.4. Decoding Microparticles.- 2.5. Statistical Analysis for Duplications.- Chapter 3. Application: Screening of Sequential Drug Combinations.- 3.1. Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways.- 3.2. Screening of Sequential Drug Combination Using a Partipetting Platform.- 3.3. Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip.- 3.4. Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin against Triple Negative Breast Cancer (TNBC).- Chapter 4. Conclusion and Discussion.- Bibliography.