1. An introduction to advanced technologies in synthetic biologyVijai Singh, Gargi Bhattacharjee, Nisarg Gohil, Rupesh Maurya, Navya L. Lam and Khalid J. Alzahrani2. Design and characterization of synthetic promotersBoris Kirov and Slavil Peykov3. Recent development in DNA synthesis technologyIndra Mani4. Advances, challenges, and opportunities in DNA sequencing technologyFrancisco J. Enguita and Ana Lucia Leitao5. Recent developments in genome design and assembly toolsCarlos Barreiro and Carlos Garcia-Estrada6. Design, building, and challenges in synthetic genomicsMansoor A. Siddiqui, Ashima Sharma and Syed Shams Yazdani7. Synthetic minimal cells and their applicationsAli Yazdizadeh Kharrazi, Amir Zare, Nils Chapin, Seyedkhashayar Ghavami and Amir Pandi8. Small RNA-based systems for sensing and therapeutic applicationsRobert Penchovsky, Georgi Y. Miloshev, Nikolet Pavlova, Katya B. Popova, Aikaterini Valsamatzi-Panagiotou, Lozena A. Otcheva and Martina Traykovska9. High-throughput navigation of the sequence spaceAlaksh Choudhury10. Advances in protein engineering and its application in synthetic biologyRongming Liu, Liya Liang, Maria Priscila Lacerda, Emily F. Freed and Carrie A. Eckert11. Computational tools for design of synthetic genetic circuitsArchit Devarajan, Dhwani Gupta, Kushika Mitra, Shalini S. Deb and Shamlan M.S. Reshamwala12. Computational tools for synthetic gene optimizationNumaan Cheema, Georgios Papamichail and Dimitris Papamichail13. De novo design and synthesis of biomoleculesSubha Sankar Paul, Debarun Dhali, Yazen Yaseen, Upasana Basu, Shilpa Pal, Nihal Mohammed and Heykel Trabelsi14. A retrobiosynthetic approach for production, conversion, sensing, dynamic regulation and degradation of moleculesPablo Carbonell15. Advances in engineering of bacteriophages for therapeutic applicationsChristian Møller-Olsen, Gurneet K. Dhanoa, Tamas Feher and Antonia P. Sagona16. Design of synthetic biology for the detection of microorganismsDong-Dong Yang, Jake Flood, Rongming Liu and Susu He17. Design of synthetic biological devices for detection and targeting human diseasesNedim Haciosmanoglu, Sila Kose, Julian Ostaku, Ilkay Cisil Koksaldi, Behide Saltepe and Urartu Ozgur Safak Seker18. Engineering cell-based therapiesJuan Luis Blazquez-Roman, Mara Pisani and Velia Siciliano19. Development of next-generation diagnostic tools using synthetic biologyElizabeth J. Vojvoda, Logan R. Burrington and Javin P. Oza20. Advances and application of CRISPR-Cas systemsRongming Liu, Liya Liang, Margaret Habib, Emily F. Freed and Carrie A. Eckert21. Synthetic biology for smart drug biosynthesis and deliverySrinivasan Ganesh Kumar, Ashok Ganesan and Shashi Kumar22. Design and applications of self-assembled soft living materials using synthetic biologyGokce Ozkul, Merve Yavuz, Nedim Haciosmanoglu, Busra Merve Kirpat and Urartu Ozgur Safak Seker23. Synthetic gene circuits for higher-order information processingKathakali Sarkar and Sangram Bagh24. Cell-free synthetic biology as an emerging biotechnologySeok Hoon Hong and Ana Serratosa Fernandez-Baca25. Recent development and applications of xeno nucleic acidsRupesh Maurya, Nisarg Gohil, Gargi Bhattacharjee, Navya L. Lam, Khalid J. Alzahrani and Vijai Singh26. Developments in the use of microfluidics in synthetic biologyLaila A. Damiati, Safa A. Damiati and Samar Damiati27. Implications of synthetic biology research and development: a structured ethical analysisKevin Smith