Metabolism by In Vivo NMR

Contributors xi
Foreword xiii
Sir George K. Radda, Medical Research Council, UK
Section A: Background
1 Introduction 1
Robert G. Shulman
2 In Vivo NMR Spectroscopy - Techniques; Direct Detection;MRS; Kinetics and Labels; Fluxes; Concentrations 7
Robin de Graaf
3 Metabolic Control Analysis for the NMR Spectroscopist 31
David A. Fell
Section B: ¯13C and ¯31P NMR Studies ofthe Regulation of Glucose Metabolism
4 MRS Studies of the Role of the Muscle Glycogen SynthesisPathway in the Pathophysiology of Type 2 Diabetes 45
Gerald I. Shulman and Douglas L. Rothman
5 Phosphorylation of Allosteric Enzymes can serve Homeostasisrather than Control Flux: the Example of Glycogen Synthase 59
James R.A. Schafer, David A. Fell, Douglas Rothman and Robert G.Shulman
6 Regulation of Glycogen Metabolism in Muscle during Exercise73
Thomas B. Price
7 ¯13C NMR Studies of Heart Glycogen Metabolism87
Maren R. Laughlin, Douglas L. Rothman and Robert G.Shulman
8 Bioenergetics Implication of Metabolic Fluctuation duringMuscle Contraction 103
Thomas Jue
9 Lactate, Glycogen and Fatigue 125
Robert G. Shulman and Douglas L. Rothman
10 Futile Cycling in Yeast: How to Control Gluttony in the Midstof Plenty 137
Jan den Hollander and Robert G. Shulman
11 Trehalose Energetics in Yeast Spores 149
Robert G. Shulman and Jan den Hollander
12 Metabolic Networks in the Liver by ²H and¯13C NMR 159
A. Dean Sherry and Craig R. Malloy
13 Summarized Reflections on Metabolism 175
Robert G. Shulman and James R. A. Schafer
Index 185