Over the last decade, high performance Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. The high speed and high separation efficiency which can be attained using any of the various modes of HPCE has resulted in the increased use of the technique in a range of analytical environments. The procedures are, however, still in the early stages of development and several barriers remain to their adoption as the technique of choice for a range of analytical problems. One such barrier is the selection and optimization of the conditions required to achieve reproducible separations of analytes and it is in this area that this new book seeks to give assistance.
The book is written by an international team of authors, drawn from both academic and industrial users, and the manufacturers of instruments. At its heart are a number of tables, divided into specific application areas. These give details of published separations of a wide range of archetypal analytes, the successful separation conditions and the matrix in which they were presented. These tables are based on separations reported since 1992 and are fully referenced to the original literature. The tables are supported by discussions of the problems that a particular area presents and the strategies and solutions adopted to overcome them. The general areas covered are biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis and environmental science.
Sprache
Verlagsort
Verlagsgruppe
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Dateigröße
ISBN-13
978-94-009-1561-9 (9789400915619)
DOI
10.1007/978-94-009-1561-9
Schweitzer Klassifikation
I.1 Electroosmotic flow (EOF) and analyte mobilization.- I.2 Separation modes and samples.- References.- One Equipment Systems.- 1 Sample injection.- 2 Quantitative analysis, precision and reproducibility.- 3 Precision and quantitation in capillary electrophoresis.- 4 Column technology.- 5 Electrolyte systems.- 6 Detection.- 7 Recent advances in fluorescence detection technology.- 8 Other systems.- 9 Advanced systems.- 10 Automated fraction collection in capillary electrophoresis.- 11 Comparison with other analytical methods.- Two Biochemistry Applications.- 12 Amino acids.- 13 Sodium dodecyl sulfate (SDS)-non-gel molecular sieving capillary electrophoresis for proteins.- 14 Protein analysis by capillary electrophoresis.- 15 Analysis of erythropoietin by capillary electrophoresis.- 16 Assay of enzymes by capillary electrophoresis.- 17 Capillary electrophoresis-based immunoassays.- 18 Analysis of antibodies by capillary electrophoresis.- 19 Analysis of peptide hormones and model peptides by capillary electrophoresis.- 20 Affinity analysis by capillary electrophoresis.- 21 Analysis of nucleic acids by capillary electrophoresis.- Three Pharmaceutical Science Applications.- 22 Analysis of pharmaceuticals by capillary electrophoresis.- 23 Analysis of medicinal plants: comparison of capillary electrophoresis with high performance liquid chromatography.- 24 Quantitative applications of the resolution of enantiomers by capillary electrophoresis.- 25 Applications of chiral capillary electrophoresis (cyclodextrin-capillary zone electrophoresis; CD-CZE).- 26 Separation of enantiomeric compounds by micelle-mediated capillary electrokinetic chromatography.- 27 Therapeutic drug monitoring by capillary electrophoresis.- Four Bioscience Applications.- 28 Biomedical applications and biological systems.- 29 Analysis of selected common clinical tests by capillary electrophoresis.- 30 Gene analysis and nucleic acid sequencing.- 31 Gene mutation and DNA sequencing.- 32 Analysis of body fluids: urine, blood, saliva and tears.- 33 Toxins associated mainly with uremia and cancer.- Five Ion Analysis Applications.- 34 Capillary electrophoresis of metal complexes.- 35 Metal chelation.- 36 Inorganic ions.- 37 Organic acids and organic ions.- Six Food Analysis Applications.- 38 Food analysis by capillary electrophoresis.- 39 Capillary zone electrophoresis analysis of additives in food samples.- 40 Analysis of underivatized carbohydrates by capillary electrophoresis and electrochemical detection.- Seven Environmental Science Applications.- 41 Hydrocarbons.- 42 Environmental pollutants.- 43 Analysis of synthetic polymers by capillary electrophoretic techniques.- Appendix A Commercially available instrumentation for capillary electrophoresis.- Appendix A1 Overview of commercial instruments T. Wehr and M. Zhu.- A1.1 Introduction.- A1.2 Power supply.- A1.3 Injection.- A1.4 Capillary temperature control.- A1.5 Liquid handling.- A1.6 Autosamplers.- A1.7 Detectors.- A1.7.1 UV-vis absorbance.- A1.7.2 Fluorescence.- A1.7.3 Other detectors.- A1.7.4 On-line coupling with mass spectrometry (MS).- A1.8 Fraction collection.- Appendix A2 The CAPI series of instruments K. Kitagishi.- A2.1 CAPI-3100.- A2.1.1 Specifications.- A2.1.2 Features.- A2.2 CAPI-1000.- A2.2.1 Specifications.- A2.2.2 Features.- Appendix B Troubleshooting K. Kitagishi.- Appendix C Commercially available buffer reagents H. Shintani.
