Mass Spectrometry for Microbial Proteomics

Section 1: Microbial Characterisation; the Transition from conventional methods to Proteomics.
(1) Changing concepts in the characterisation of microbes - H. N. Shah (HPA).
(2) Microbial phylogeny and evolution; the change from targeted genes to proteins - Radhey Gupta (Canada),.
.
Section 2: Mass Spectrometers and Specific Applications.
(3) Range of instruments; criteria for selection for microbial analysis. - Robert Parker (HPA).
(4) Tools for rapid Mass spectral analysis (Bob Galvin (ABI).
(5) Hybrid Mass Spectrometers - Michaela Scigelova (Thermo).
Section 3: Protein Samples Preparation Techniques.
(6) Conventional approaches for sample preparation and 2-D gel electrophoresis applications: Vesela Encheva (HPA).
(7) Protein fractionation and analysis using multidimensional chromatography (PF2D).- Jim Thorn, BeckmanCoulter Inc.
(8) On-Chip sample preparation and concentration using newly developed surfaces prior to high-sensitivity MALDI analysis. Winfred Girg (Qiagen).
(9) News affinity capture methods: David O'Connor (Univ. Southampton)..
Section 4: Characterisation of microorganisms by Pattern Matching of Mass Spectral Profiles and Biomarker Approaches Requiring Minimal Sample Preparation.
(10) Analysis of Surface-Associated Molecules of Intact Bacterial Cells using.
MALDI- TOF-MS - Diane Dare.
(11) Analysis based upon Membrane-Bound/Intracellular Protein Patterns using.
SELDI-TOF-MS - H. N. Shah et al.(MISU).
(12) Microbial Disease Biomrakers using ProteinChip Arrays -Paul Langford,.
Imperial.
(13) Rapid identification of microorganisms using stable intracellular proteins.
Markus Kostrzewa & Wolfgang Pusch, Brucker, Germany.
*Plus additional chapter on viruses (Catherine Fenselau to be invited).
Section 5: Targeted Molecules and Analysis of Specific Microorganisms.
(14) Analysis of High risk pathogens - Ben van Baar (Holland).
(15) Proteome Analysis of E. coli - Robin Wait (Imperial College).
(16) Comparative DNA sequence analysis and typing using Mass Spectrometry.
- Christiane Honisch & Yong Chen; SEQUENOM Inc. (USA).
(17) Analysis based upon whole cell digestion; comparative proteomic of.
antibiotic resistant and sensitive strains of Staphylococcus aureus. Therese.
McKenna et al.
Section 6: Data Analysis.
(18) Artificial Neural Networks of Mass Spectral Data - Graham Ball (Nottingham Trent University).
(19) Devising a platform for microbial identification and comparative.
analysis: Martin Lund (Waters).
(20) Analysis of 2-D gels using DeCyder EDA (Extended Data Analysis).
Matthias Berg, Germany.
* Plus two additional chapters from Jerry Thomas (University of York) and Natasha Karp (University of Cambridge)