In this monograph all presently available methods for quantitative measurement of calcium-regulating hormones, vitamin D metabolites, and cAMP as well as immunochemiluminescence and adenyl cyclase bioassays are described. This is a practically and clinically oriented monograph for working in the fields of internal medicine, endocrinology, and laboratory medicine.
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
24 s/w Abbildungen
XI, 370 p. 24 illus.
Dateigröße
ISBN-13
978-3-662-00406-7 (9783662004067)
DOI
10.1007/978-3-662-00406-7
Schweitzer Klassifikation
1 Introduction.- 1.1 Clinical Application of Intact Parathyroid Hormone Determination.- 1.2 Clinical Use of Vitamin D Metabolite Assays (Calcidiol and Calcitriol).- 1.3 Diagnostic Value of the Peptides from the Calcitonin Gene.- 1.4 Immunoassay Vial for Improved Assay Performance.- 2 Parathyroid Hormone Assays and Clinical Application.- 2.1 Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53-84) with Tyrosylated Peptide as Tracer.- 2.2 Sensitive Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53-84) to Diagnose Hypoparathyroid Conditions: Guidelines for the Establishment of Highly Sensitive Assays.- 2.3 Rapid Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53-84) as a Diagnostic Aid in Hypercalcemic Crisis.- 2.4 Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 44-68) with Tyrosylated Peptide as Tracer.- 2.5 Purification of 125Iodine-Labeled Tyrosylated Human Parathyroid Hormone Fragment (Residues 44-68), Optimized by Reverse-Phase High-Performance Liquid Chromatography.- 2.6 Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 28-48) with Tyrosylated Peptide as Tracer.- 2.7 Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 1-34) with Biotinylated Peptide as Tracer.- 2.8 Measurement of Intact Parathyroid Hormone by an Extracting Two-Site Immunoradiometric Assay.- 2.9 Measurement of Intact Parathyroid Hormone by a Two-Site Immunochemiluminometric Assay.- 2.10 A Sensitive Adenylate Cyclase Bioassay for Parathyroid Hormone.- 2.11 Immunological Properties of Asparagine versus Aspartic Acid at Residue 76 of Human Parathyroid Hormone (Residues 53-84).- 3 Parathyroid Hormone-Related Protein.- 3.1 Parathyroid Hormone-Related Protein.- 4 Cyclic AMP.- 4.1Plasma and Urinary Cyclic AMP Assay and Clinical Application.- 5 Vitamin D: Assays of 25-Hydroxyvitamin D (Calcidiol) and 1,25-Dihydroxyvitamin D (Calcitriol).- 5.1 Simultaneous Determination of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 by High-Performance Liquid Chromatography.- 5.2 Competitive Protein-Binding Assay for the Diagnosis of Hyper- and Hypovitaminosis D.- 5.3 A Competitive Protein-Binding Assay with Second Antibody Separation for the Diagnosis of Hyper- and Hypovitaminosis D.- 5.4 Interlaboratory Comparison of Calcidiol Determination.- 5.5 A Sensitive Radioimmunoassay for 1,25-Dihydroxyvitamin D3 (Calcitriol) after High Performance Liquid Chromatography of Plasma or Serum Extracts.- 5.6 A Double Antibody Radioimmunoassay for measurement of 1?, 25-Dihydroxyvitamin D3.- 5.7 Competitive Protein-Binding Assay of Calcitriol with an Advanced Preparation of Bovine Calf Thymus Cytosol.- 6 Assay of Calcitonin and Related Peptides.- 6.1 Design of New Methods for Measuring Calcitonin and Related Peptides Using Monoclonal Antipeptide Antibodies.
