Single-channel recording has become a widely used tool for the study of ion permeation mechanisms in biological membranes. Whereas the technique might have been considered an "art" after its introduction in 1976, it developed into a relatively simple method after it became possible to obtain high-resistance (several gigaohm) membrane-pipette seals. In the summer of 1982, a course on the technique was held at the Ettore Majorana Center for Scientific Culture in Erice, Sicily. It brought together people from most of the laboratories involved in patch clamping at that time. During the course, it became apparent that the technique had reached a state of maturity. Repeatedly, the opinion was expressed that a detailed description of all the aspects of the technique including representative examples of results should be available. We therefore asked the course instructors, as well as several other colleagues, to provide chapters on selected topics in order to produce this volume. The different variants of patch clamping were described quite extensively in an article by Hamill, Marty, Neher, Sakmann, and Sigworth (Pflugers Archiv 391:85) in 1981. Rather than repeating this survey in an introductory chapter, we chose to reprint that article in the Appendix of this volume (by permission of Springer-Verlag). The methods section will, therefore, go straight into detailed aspects of the technol ogy.
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
136 s/w Abbildungen
XXII, 504 p. 136 illus.
Dateigröße
ISBN-13
978-1-4615-7858-1 (9781461578581)
DOI
10.1007/978-1-4615-7858-1
Schweitzer Klassifikation
I. Methods.- 1 Electronic Design of the Patch Clamp.- 2 Geometric Parameters of Pipettes and Membrane Patches.- 3 Science and Technology of Patch-Recording Electrodes.- 4 Enzymatic Dispersion of Heart and Other Tissues.- 5 A Primer in Cell Culture for Patchologists.- 6 Patch-Clamped Liposomes: Recording Reconstituted Ion Channels.- 7 Tight-Seal Whole-Cell Recording.- 8 The Loose Patch Clamp.- II. Concepts and Analysis.- 9 The Principles of the Stochastic Interpretation of Ion-Channel Mechanisms.- 10 Conformational Transitions of Ionic Channels.- 11 Fitting and Statistical Analysis of Single-Channel Records.- 12 Automated Analysis of Single-Channel Records.- 13 Analysis of Nonstationary Channel Kinetics.- 14 An Example of Analysis.- 15 Membrane Current and Membrane Potential from Single-Channel Kinetics.- III. Patch Clamp Data.- 16 Bursts of Openings in Transmitter-Activated Ion Channels.- 17 Is the Acetylcholine Receptor a Unit-Conductance Channel?.- 18 Analysis of Single-Channel Data from Glutamate Receptor-Channel Complexes on Locust Muscle.- 19 Experimental Approaches Used to Examine Single Glutamate-Receptor Ion Channels in Locust Muscle Fibers.- 20 Cholinergic Chloride Channels in Snail Neurons.- 21 Single-Channel Analysis in Aplysia Neurons: A Specific K + Channel is Modulated by Serotonin and Cyclic AMP.- 22 Cholecystokinin and Acetylcholine Activation of Single-Channel Currents via Second Messenger in Pancreatic Acinar Cells.- 23 Observations on Single Calcium Channels: An Overview.- 24 Potassium and Chloride Channels in Red Blood Cells.- 25 The Influence of Membrane Isolation on Single Acetylcholine-Channel Current in Rat Myotubes.
