Edited by the 1991 winners of the Nobel Prize in Physiology or Medicine, this
Second Edition
includes new chapters covering such applications as capacitance measurements; single-cell PCR measurements; whole-cell recording from brain slices in combination with imaging techniques; atomic force microscopy of cells and membranes attached to glass pipettes; and patch clamping.
Rezensionen / Stimmen
From reviews of the First Edition:
"A landmark in the field of membrane biophysics." (Science)
"A first-class laboratory guide...an indispensable reference." (Neuroscience)
Auflage
Sprache
Verlagsort
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Illustrationen
2
50 s/w Abbildungen, 2 farbige Abbildungen
XXII, 700 p. 52 illus., 2 illus. in color.
Dateigröße
ISBN-13
978-1-4419-1229-9 (9781441912299)
DOI
10.1007/978-1-4419-1229-9
Schweitzer Klassifikation
to Patch Clamping.- A Practical Guide to Patch Clamping.- Tight-Seal Whole-Cell Recording.- Guide to Data Acquisition and Analysis.- Methods.- Electronic Design of the Patch Clamp.- Low-Noise Recording.- Voltage Offsets in Patch-Clamp Experiments.- Techniques for Membrane Capacitance Measurements.- Patch-Pipette Recordings from the Soma, Dendrites, and Axon of Neurons in Brain Slices.- Patch Clamp and Calcium Imaging in Brain Slices.- Fast Application of Agonists to Isolated Membrane Patches.- Electrochemical Detection of Secretion from Single Cells.- Technical Approaches to Studying Specific Properties of Ion Channels in Plants.- The Giant Membrane Patch.- A Fast Pressure-Clamp Technique for Studying Mechanogated Channels.- Electrophysiological Recordings from Xenopus Oocytes.- Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices.- Force Microscopy on Membrane Patches.- Analysis.- The Principles of the Stochastic Interpretation of Ion-Channel Mechanisms.- Fitting and Statistical Analysis of Single-Channel Records.- A Q-Matrix Cookbook.- "Classics".- Geometric Parameters of Pipettes and Membrane Patches.- Conformational Transitions of Ionic Channels.
