Epitope Mapping Protocols

An Overview of Epitopes and Methods for Antibody Epitope Mapping.- Antibody-Antigen Epitope Mapping by X-Ray Crystallography.- Mapping Antibody Epitopes by Solution NMR Spectroscopy: Practical Considerations.- Cryo-Electron Microscopy-Based Epitope Mapping.- Epitope Mapping Using Yeast Display and Next Generation Sequencing.- Rapid Mapping of Protein Binding Sites and Conformational Epitopes by Coupling Yeast Surface Display to Chemical Labeling and Deep Sequencing.- High-Density Antigen Microarrays for the Assessment of Antibody Selectivity.- Mammalian Cell Display with Automated Oligo Design and Library Assembly Allows for Rapid Residue Level Conformational Epitope Mapping.- Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries.- Mapping of Antibody Structural Epitopes Using Bacterial Cell Surface Display of Folded Domains.- Highly Multiplexed Immunoassays for the Validation of Anti-GPCR Antibodies.- Probing Antibody Binding Sites on G Protein-Coupled Receptors Using Genetically-Encoded Photo-Activatable Cross-Linkers.- Genetic Code Expansion to Enable Site-Specific Post-Translational Peptide Epitope Tagging of G Protein-Coupled Receptors in Live Cells.- Multiplexed Epitope Mapping of HIV-1 Neutralizing Antibodies by Coupling Chemical Labeling of Cysteines to Deep Sequencing.- Predicting Epitopes of Synthetic Binding Proteins Using Protein Structure Prediction and Protein-Protein Docking.- Structure-Based Epitope Profiling with the Structural Profiling of Antibodies to Cluster by Epitope 2 (SPACE2) Algorithm.- Binding Kinetics and Epitope Binning Using Surface Plasmon Resonance.- High-Throughput Epitope Binning Analysis of Human Monoclonal Antibodies to Assess Epitope Diversity Using the Octet Bio-Layer Interferometry Platform.- Competitive Epitope Binning Using HT-SPR.- Label-Free Cell-Based Assay for Characterization of Biomolecules and Receptors.- B Cell Epitope Mapping by High Density Competition ELISAs.