This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in
Saccharomyces cerevisiae
, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Trinucleotide Repeats: Methods and Protocols
serves as avaluable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
12
53 farbige Abbildungen, 12 s/w Abbildungen
XI, 286 p. 65 illus., 53 illus. in color.
Dateigröße
ISBN-13
978-1-4939-9784-8 (9781493997848)
DOI
10.1007/978-1-4939-9784-8
Schweitzer Klassifikation