Electrofocusing and Isotachophoresis

Name: Electrofocusing and Isotachophoresis | Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany
Brand: De Gruyter
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Proceedings of the International Symposium, August 1-4, 1976, Hamburg, Germany

B. J. Radola D. Graesslin(Herausgeber*in)

De Gruyter (Verlag)

1. Auflage

Erschienen am 22. Juli 2019

XVI, 608 Seiten

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Inhalt

Intro
Preface
Contents
Contributors
Part I. Isoelectric Focusing
1. Theoretical and General Aspects
Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea
Stable pH Gradients - A Key Problem in Isoelectric Focusing
Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential
Comparison of pH Gradients Used for Isoelectric Focusing
On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing
2. Carrier Ampholytes
Carrier Ampholyte Distribution
Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods
Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range
3. Analytical Methodology
Staining of Proteins in Polyacrylamide Gels
Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF
Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing
Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry
Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins
A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis
pH Determinations in Isoelectric Focusing with an Iridium Electrode
Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing
Does Lipoprotein Lipase Bind Ampholytes?
4. Biochemical Applications
Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation
Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems
A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing
Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae)
Fractionation of Nucleic Acids on Isoelectric Focusing
5. Clinical Applications
Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (a⊂&1 &/subΠ) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies
Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (a⊂&1 &/subΠ) Phenotyping: II. Immunochemical Procedures
High Resolution Phenotyping of a⊂&1&/sub&-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing
Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation
Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing
The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing
Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality
Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing
Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay
Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing
A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing
6. Two-Dimensional Techniques
Biological Applications of Two-Dimensional Gel Electrophoresis
Plant Proteins Evaluated by Two-Dimensional Methods
Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis
Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins
Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens
7. Preparative Separations
Isoelectric Focusing in Free Water
Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects
Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis
Preparative Zone Convection Electrofocusing in Helical Glass Tubes
Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential
8. Separation of Cells
New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques
Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction
Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient
Part II. Isotachophoresis
1. Analytical and Preparative Methodology
The Principle of Preparative Capillary Isotachophoresis
Preparative Capillary Isotachophoresis: Separation of µg Amounts of Some Human Serum Proteins
Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis
Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing
Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization
2. Applications
Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers
Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis
Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies
Index

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