Biophysical Approaches for the Study of Membrane Structure Part A

PrefaceTobias Baumgart and Markus Deserno1. Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging FCSNirmalya Bag, Arpita Tripathy and Sudipti Priyadarsinee2. Fluorescence imaging of lamellipodin mediated biomolecular condensates on solid supported lipid bilayer membranesTobias Baumgart3. Effects of lateral and hydrostatic pressure on membrane structure and propertiesNick Brooks4. Using the yeast vacuole as a system to test the lipid drivers of membrane heterogeneity in living cellsItay Budin, Israel Juarez-Contreras and Hyesoo Kim5. The spectral phasor approach to resolving membrane order with environmentally sensitive dyesRumiana Dimova and Agustín Mangiarotti6. The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domainsThais A. Enoki7. Advanced microscopy methods to study membrane poresAna J. García-Sáez8. Use of cryo-EM to study membrane phase separationFrederick Heberle and M. Neal Waxham9. Using lipid binding proteins and advanced microscopy to study lipid domainsTOSHIHIDE KOBAYASHI, NARIO TOMISHIGE, KOHTA TAKAHASHI, BRIGITTE POLLET, LUDOVIC RICHERT and YVES MÉLY10. Structural characterization of lateral phase separation in polymer-lipid hybrid membranesCecília Leal, Nurila Kambar, Minh N. Do, Corey Snyder and Yoo Kyung Go11. Applications of phase-separating multi-bilayers in protein-membrane domain interactionsKandice R. Levental and Hong-Yin Wang12. Studying lipid flip-flop using NMR and TR-SANSDrew Marquardt, Dominik Dziura and Maksymilian Dziura13. Thickness determination of hydroperoxidized lipid bilayers from medium-resolution cryo-TEM imagesCarlos Marques, André Schroder, Marc Schmutz, Pierre Muller and Eulalie J. Lafarge14. Structure of symmetric and asymmetric lipid membranes from joint SAXS/SANSGeorg Pabst, Enrico Semeraro and Moritz Frewein15. Quantification of membrane geometry and protein sorting on cell membrane protrusions using fluorescence microscopyZheng Shi and Shilong Yang16. A TIRF microscopy-based method for observing the uptake of individual particles by clathrin mediated endocytosisJeanne Stachowiak, Grant Ashby, Kayla Keng and Carl Hayden17. Pore-spanning membranes as a tool to investigate lateral lipid membrane heterogeneityClaudia Steinem and Larissa Socrier18. MaCD based plasma membrane outer leaflet lipid exchange in mammalian cells to study insulin receptor activityPavana Suresh and Erwin London