This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and fully updated,
Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition
serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development.
Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Reihe
Auflage
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
70
12 s/w Abbildungen, 70 farbige Abbildungen
XI, 434 p. 82 illus., 70 illus. in color.
ISBN-13
978-1-4939-9009-2 (9781493990092)
DOI
10.1007/978-1-4939-9009-2
Schweitzer Klassifikation
