Apobec Enzymes

Section 1: Assays to characterize DNA or RNA editing activity1. Fluorescent shift assay for APOBECs RNA editingXiaojiang S. Chen2. Low Error Sequencing Methods to Tetect APOBEC-mediated RNA editing: Circular RNAseq and Safe-Sequencing SystemXiaojiang S. Chen3. "Safe-Barcode" RNAseq assay for APOBECs RNA editingXiaojiang S. Chen4. DT40 cell system to characterize somatic hypermutationSilvestro Conticello5. CH12 cell system to assay AID activity on class switch recombinationSilvestro Conticello6. Purification of Enzymatically Active APOBEC Proteins from an Insect Cell Expression SystemLinda Chelico7. In vitro deamination assay to measure the activity and processivity of AID/APOBEC deaminasesLinda Chelico8. Defining genome-wide mutagenic impact of APOBEC3 enzymesAbby M. Green and Dávid Szüts9. APOBEC-induced mutational assay in yeastSteven Andrew RobertsSection 2: Assays for APOBEC drug discovery10. Biochemical assay for the identification of APOBECs inhibitorsMani LarijaniSection 3: monitoring RNA or DNA editing11. An In Vitro Cytidine Deaminase Assay to Monitor APOBEC activity on DNARémi Buisson12. Profiling rare C-to-U editing events via direct RNA sequencingErnesto Picardi and Adriano Fonzino13. Global quantification of off-target activity by base editorsErez Levanon and Michelle EidelmanSection 4: Deaminases as tools for programmable RNA or DNA base editing14. Programmable C to U RNA editing based on the APOBEC1 deaminaseToshifumi TsukaharaSection 5: Deaminases as tools for molecules tracking15. Harnessing APOBEC editing for detecting RBP-RNA interactionsGene W. Yeo and Tao Yu16. Programmable C to U editing to track endogenous proteinsTao Liu