Neural Stem Cells

Flow-Cytometry-Based Protocols for the Mouse Subependymal Neurogenic Niche Phenotyping.- Isolation of Adult Hippocampal Neural Stem Cells.- Low Density Primary Cell Culture of Postnatal Murine Cerebellar Progenitors In Vitro.- Isolation of Oligodendrocyte Precursor Cells with Immunomagnetic Beads for Their Better Viability In Vitro and In Vivo.- Non-Hematopoietic Stem Cell Identification and Sorting in Adult Retina.- Neurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay.- Detection of Monocytic Myeloid-Derived Suppressor Cells in the Human Brain and Blood.- Cell-Type Specific Protein Metabolic Labeling and Identification Using the Methionine Subrogate ANL in Cells Expressing a Mutant Methionyl-tRNA Synthetase.- Experimental Neurogenesis in the Embryos of the Gecko Paroedura picta.- Genetic Tracing of Progenitors from Embryo to Postnatal Brain.- Tracking Neural Migration in Dentate Gyrus Using Thymidine Analogous.- Electroporation Techniques to Target Subependymal Zone Neurogenic Niche.- In Vitro and In Vivo Direct Reprogramming of Astrocytes to Induced-Neurons.- Retrovirus-Mediated Reprogramming of Endogenous Hippocampal Glia into GABAergic Induced Neurons.- Gene Expression Manipulation via Acute Electroporation in Human Brain Organoids.- Targeted Human Editing Using CRISPR Technology in Neural Stem Cells.- Patch-Clamp Recordings in Isolated Neural Cells, Acute Tissue Slices, and Brain Rosettes.- Acute Slice Patch-Clamp Recordings to Characterize Neural Populations in the Hippocampal Subgranular Zone.- Intracellular Calcium Recording and Immunostaining of Single Neural Populations In Vitro.- Cell Population Analyses of Neurospheres Generated In Vitro from Ovarian Cortical Cells in Mammals: Characterization by Immunohistochemistry and Flow Cytometry.- Reference-Based Gene Expression Analysis Using Galaxy Server.- Development of Novel Neuroprotective Agents Targeting GPCR Using In Vitro Assays.- Picrotoxin-Induced Epileptic Hippocampal Organotypic Slice Cultures (hOTCs).